BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16075

Title: Mouse prion protein fragment mPrP[M129V](91-231) at pH 7.0   PubMed: 19546219

Authors: Damberger, Fred; Wuthrich, Kurt; Hornemann, Simone; von Schroetter, Christine

Citation: Hornemann, Simone; von Schroetter, Christine; Damberger, Fred; Wuthrich, Kurt. "Prion protein-detergent micelle interactions studied by NMR in solution."  J. Biol. Chem. 284, 22713-22721 (2009).

Assembly members:
mPrP90_M129V, polymer, 141 residues, 16093.8 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mPrP90_M129V: QGGGTHNQWNKPSKPKTNLK HVAGAAAAGAVVGGLGGYVL GSAMSRPMIHFGNDWEDRYY RENMYRYPNQVYYRPVDQYS NQNNFVHDCVNITIKQHTVT TTTKGENFTETDVKMMERVV EQMCVTQYQKESQAYYDGRR S

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mPrP90_M129V1

Entities:

Entity 1, mPrP90_M129V 141 residues - 16093.8 Da.

1   GLNGLYGLYGLYTHRHISASNGLNTRPASN
2   LYSPROSERLYSPROLYSTHRASNLEULYS
3   HISVALALAGLYALAALAALAALAGLYALA
4   VALVALGLYGLYLEUGLYGLYTYRVALLEU
5   GLYSERALAMETSERARGPROMETILEHIS
6   PHEGLYASNASPTRPGLUASPARGTYRTYR
7   ARGGLUASNMETTYRARGTYRPROASNGLN
8   VALTYRTYRARGPROVALASPGLNTYRSER
9   ASNGLNASNASNPHEVALHISASPCYSVAL
10   ASNILETHRILELYSGLNHISTHRVALTHR
11   THRTHRTHRLYSGLYGLUASNPHETHRGLU
12   THRASPVALLYSMETMETGLUARGVALVAL
13   GLUGLNMETCYSVALTHRGLNTYRGLNLYS
14   GLUSERGLNALATYRTYRASPGLYARGARG
15   SER

Samples:

sample_1: mPrP90_M129V, [U-99% 13C; U-99% 15N], 1.2 ± 0.05 mM; sodium phosphate buffer 5 mM

sample_conditions_1: ionic strength: 0.03 M; pH: 7.0; pressure: 1 atm; temperature: 293.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15845 16071 16076 16077 16078 16079 16080 16185 16722 17081 17082 17084 17174 17213 17758
PDB
DBJ BAA08790 BAE28320 BAE28693 BAE29994 BAE34221
EMBL CAJ18553
GB AAA39996 AAA39997 AAA39998 AAA39999 AAA41947
PRF 1106228A
REF NP_001265185 NP_035300 NP_036763 XP_006235124 XP_006984054
SP P04925 P13852 Q9Z0T3