BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16063

Title: Solution structure of ILK/PINCH complex   PubMed: 19117955

Authors: Qin, Jun

Citation: Yang, Yanwu; Wang, Xiaoxia; Hawkins, Cheryl; Chen, Kan; Vaynberg, Julia; Mao, Xian; Tu, Yizeng; Zuo, Xiaobing; Wang, Jinbu; Wang, Yun-xing; Wu, Chuanyue; Tjandra, Nico; Qin, Jun. "Structural Basis of Focal Adhesion Localization of LIM-only Adaptor PINCH by Integrin-linked Kinase"  J. Biol. Chem. 284, 5836-5844 (2009).

Assembly members:
ILK, polymer, 171 residues, 19380.070 Da.
PINCH, polymer, 70 residues, 7963.068 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ILK: MDDIFTQCREGNAVAVRLWL DNTENDLNQGDDHGFSPLHW ACREGRSAVVEMLIMRGARI NVMNRGDDTPLHLAASHGHR DIVQKLLQYKADINAVNEHG NVPLHYACFWGQDQVAEDLV ANGALVSICNKYGEMPVDKA KAPLRELLRERAEKMGQNLN RIPYKDTFWKG
PINCH: MANALASATCERCKGGFAPA EKIVNSNGELYHEQCFVCAQ CFQQFPEGLFYEFEGRKYCE HDFQMLFAPC

Data sets:
Data typeCount
13C chemical shifts583
15N chemical shifts190
1H chemical shifts1211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ILK1
2PINCH2
3ZINC ION_13
4ZINC ION_23

Entities:

Entity 1, ILK 171 residues - 19380.070 Da.

1   METASPASPILEPHETHRGLNCYSARGGLU
2   GLYASNALAVALALAVALARGLEUTRPLEU
3   ASPASNTHRGLUASNASPLEUASNGLNGLY
4   ASPASPHISGLYPHESERPROLEUHISTRP
5   ALACYSARGGLUGLYARGSERALAVALVAL
6   GLUMETLEUILEMETARGGLYALAARGILE
7   ASNVALMETASNARGGLYASPASPTHRPRO
8   LEUHISLEUALAALASERHISGLYHISARG
9   ASPILEVALGLNLYSLEULEUGLNTYRLYS
10   ALAASPILEASNALAVALASNGLUHISGLY
11   ASNVALPROLEUHISTYRALACYSPHETRP
12   GLYGLNASPGLNVALALAGLUASPLEUVAL
13   ALAASNGLYALALEUVALSERILECYSASN
14   LYSTYRGLYGLUMETPROVALASPLYSALA
15   LYSALAPROLEUARGGLULEULEUARGGLU
16   ARGALAGLULYSMETGLYGLNASNLEUASN
17   ARGILEPROTYRLYSASPTHRPHETRPLYS
18   GLY

Entity 2, PINCH 70 residues - 7963.068 Da.

1   METALAASNALALEUALASERALATHRCYS
2   GLUARGCYSLYSGLYGLYPHEALAPROALA
3   GLULYSILEVALASNSERASNGLYGLULEU
4   TYRHISGLUGLNCYSPHEVALCYSALAGLN
5   CYSPHEGLNGLNPHEPROGLUGLYLEUPHE
6   TYRGLUPHEGLUGLYARGLYSTYRCYSGLU
7   HISASPPHEGLNMETLEUPHEALAPROCYS

Entity 3, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1, [U-100% 15N], 0.1-0.5 mM; entity_1, [U-100% 13C; U-100% 15N], 0.1-0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAG73298 BAH12404 BAH56666 BAG35111 BAG60588 BAH14581
EMBL CAB94832 CAB99253 CAG28601 CAH18077 CAH92862
GB AAB94646 AAC16892 AAF70501 AAF74449 AAH01554 AAF99328 AAH93812 AAI12234 AAY14903 AAY24131
PRF 2202330A
REF NP_001014794 NP_001014795 NP_001029865 NP_001126674 NP_001155196 NP_001192217 NP_277049 XP_002811796 XP_002929430 XP_003309226
SP O55222 P57044 Q13418 Q3SWY2 Q5R5V4 P0CW19 P0CW20
TPG DAA22142
BMRB 4884