BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16062

Title: NMR solution structure of the pheromone En-1 of Euplotes nobilii at -1.5 C   PubMed: 19621350

Authors: Pedrini, Bill; Alimenti, Claudio; Vallesi, Adriana; Luporini, Pierangelo; Wuthrich, Kurt

Citation: Alimenti, Claudio; Vallesi, Adriana; Pedrini, Bill; Wuthrich, Kurt; Luporini, Pierangelo. "Molecular cold-adaptation: Comparative analysis of two homologous families of psychrophilic and mesophilic signal proteins of the protozoan ciliate, Euplotes"  IUBMB Life 61, 838-845 (2009).

Assembly members:
En-1, polymer, 52 residues, 5630.097 Da.

Natural source:   Common Name: Euplotes nobilii   Taxonomy ID: 184062   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Euplotes nobilii

Experimental source:   Production method: purified from the natural source   Host organism: Euplotes nobilii

Entity Sequences (FASTA):
En-1: NPEDWFTPDTCAYGDSNTAW TTCTTPGQTCYTCCSSCFDV VGEQACQMSAQC

Data sets:
Data typeCount
13C chemical shifts139
1H chemical shifts300

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1En-11

Entities:

Entity 1, En-1 52 residues - 5630.097 Da.

1   ASNPROGLUASPTRPPHETHRPROASPTHR
2   CYSALATYRGLYASPSERASNTHRALATRP
3   THRTHRCYSTHRTHRPROGLYGLNTHRCYS
4   TYRTHRCYSCYSSERSERCYSPHEASPVAL
5   VALGLYGLUGLNALACYSGLNMETSERALA
6   GLNCYS

Samples:

sample_1: En-1 1.0 mM; sodium phosphate 20 mM

sample_conditions_1: pH: 6.0; temperature: 275.0 K

sample_conditions_2: pH: 6.0; temperature: 271.7 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_2

Software:

CARA v1.4.1, Keller and Wuthrich - chemical shift assignment

ATNOS v1.2, Herrmann, Guntert, Wuthrich - peak picking

CANDID, Herrmann, Guntert and Wuthrich - distance constraint calculation, peak assignment

CYANA v1, Guntert, Mumenthaler and Wuthrich - torsion angle dynamics simulated annealing

OPALp, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB ACQ66088
SP P83441