BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16056

Title: STRUCTURAL AND FUNCTIONAL ANALYSIS OF TM XI OF THE NHE1 ISOFORM OF THE NA+/H+ EXCHANGER   PubMed: 19176522

Authors: Lee, Brian; Li, Xiuju; Liu, Yongsheng; Sykes, Brian; Fliegel, Larry

Citation: Lee, Brian; Li, Xiuju; Liu, Yongsheng; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of TM XI of the NHE1 Isoform of the Na+/H+ Exchanger"  J. Biol. Chem. 284, 11546-11556 (2009).

Assembly members:
TMXI, polymer, 28 residues, 2860.407 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
TMXI: XKDQFIIAYGGLRGAIAFSL GYLLDKKX

Data sets:
Data typeCount
1H chemical shifts208
13C chemical shifts46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TMXI1

Entities:

Entity 1, TMXI 28 residues - 2860.407 Da.

N terminal acetate cap, C terminal NH2 cap

1   ACELYSASPGLNPHEILEILEALATYRGLY
2   GLYLEUARGGLYALAILEALAPHESERLEU
3   GLYTYRLEULEUASPLYSLYSNH2

Samples:

sample_1: DPC 150 mM; DSS 0.25 mM; TMXI 2 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 303 K; pH: 5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - structure solution, refinement

VNMRJ, Varian - collection

NMRView, Johnson, One Moon Scientific - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAE29332 BAE32882 BAE38221 BAG36869 BAG62158
EMBL CAA10507 CAA42558 CAA43721 CAA69925
GB AAA91483 AAA92976 AAA98479 AAB59460 AAC60606
REF NP_001081553 NP_001095191 NP_001273957 NP_003038 NP_036784
SP P19634 P23791 P26431 Q28036 Q61165
TPG DAA32066