BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16039

Title: NMR structure of the unphosphorylated form of OdhI, OdhI.   PubMed: 19368890

Authors: Barthe, Philippe; Roumestand, Christian; Canova, Marc; Hurard, Corinne; Molle, Virginie; Cohen-Gonsaud, Martin

Citation: Barthe, Philippe; Roumestand, Christian; Canova, Marc; Kremer, Laurent; Hurard, Corinne; Molle, Virginie; Cohen-Gonsaud, Martin. "Dynamic and Structural Characterization of a Bacterial FHA Protein Reveals a New Autoinhibition Mechanism"  Structure 17, 568-578 (2009).

Assembly members:
OdhI, polymer, 143 residues, 15700.225 Da.

Natural source:   Common Name: Corynebacterium glutamicum   Taxonomy ID: 1718   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium glutamicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
OdhI: MSDNNGTPEPQVETTSVFRA DLLKEMESSTGTAPASTGAE NLPAGSALLVVKRGPNAGAR FLLDQPTTTAGRHPESDIFL DDVTVSRRHAEFRINEGEFE VVDVGSLNGTYVNREPRNAQ VMQTGDEIQIGKFRLVFLAG PAE

Data sets:
Data typeCount
15N chemical shifts145
1H chemical shifts970

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1OdhI1

Entities:

Entity 1, OdhI 143 residues - 15700.225 Da.

1   METSERASPASNASNGLYTHRPROGLUPRO
2   GLNVALGLUTHRTHRSERVALPHEARGALA
3   ASPLEULEULYSGLUMETGLUSERSERTHR
4   GLYTHRALAPROALASERTHRGLYALAGLU
5   ASNLEUPROALAGLYSERALALEULEUVAL
6   VALLYSARGGLYPROASNALAGLYALAARG
7   PHELEULEUASPGLNPROTHRTHRTHRALA
8   GLYARGHISPROGLUSERASPILEPHELEU
9   ASPASPVALTHRVALSERARGARGHISALA
10   GLUPHEARGILEASNGLUGLYGLUPHEGLU
11   VALVALASPVALGLYSERLEUASNGLYTHR
12   TYRVALASNARGGLUPROARGASNALAGLN
13   VALMETGLNTHRGLYASPGLUILEGLNILE
14   GLYLYSPHEARGLEUVALPHELEUALAGLY
15   PROALAGLU

Samples:

sample_1: OdhI, [U-100% 15N], 0.3 mM; H2O 95%; D2O 5%; NaCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16038
PDB
DBJ BAB98834 BAF54493
EMBL CAF21450 CCH24605
GB AGG66772 AGN19176 AGN22201 AGT05407 AIK85116
REF NP_600658 WP_003856253 WP_015651203
SP Q8NQJ3