BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16024

Title: NMR structrure of gallium subsituted ferredoxin   PubMed: 20690702

Authors: Xu, Xingfu; Ubbink, Marcellus; Knaff, David

Citation: Xu, Xingfu; Scanu, Sandra; Chung, Jung-Sung; Hirasawa, Masakazu; Knaff, David; Ubbink, Marcellus. "Structural and functional characterization of the ga-substituted ferredoxin from Synechocystis sp. PCC6803, a mimic of the native protein."  Biochemistry 49, 7790-7797 (2010).

Assembly members:
Ferredoxin, polymer, 96 residues, 10237.103 Da.
GA, non-polymer, 69.723 Da.

Natural source:   Common Name: Synechocystis sp. PCC 6803   Taxonomy ID: 1148   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Synechocystis Synechocystis sp. PCC 6803

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ferredoxin: ASYTVKLITPDGESSIECSD DTYILDAAEEAGLDLPYSCR AGACSTCAGKITAGSVDQSD QSFLDDDQIEAGYVLTCVAY PTSDCTIETHKEEDLY

Data sets:
Data typeCount
15N chemical shifts96
1H chemical shifts598

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ferredoxin1
2GALLIUM (III) ION2

Entities:

Entity 1, Ferredoxin 96 residues - 10237.103 Da.

1   ALASERTYRTHRVALLYSLEUILETHRPRO
2   ASPGLYGLUSERSERILEGLUCYSSERASP
3   ASPTHRTYRILELEUASPALAALAGLUGLU
4   ALAGLYLEUASPLEUPROTYRSERCYSARG
5   ALAGLYALACYSSERTHRCYSALAGLYLYS
6   ILETHRALAGLYSERVALASPGLNSERASP
7   GLNSERPHELEUASPASPASPGLNILEGLU
8   ALAGLYTYRVALLEUTHRCYSVALALATYR
9   PROTHRSERASPCYSTHRILEGLUTHRHIS
10   LYSGLUGLUASPLEUTYR

Entity 2, GALLIUM (III) ION - Ga - 69.723 Da.

1   GA

Samples:

sample_1: GaFd, [U-99% 15N], 1 mM; sodium phosphate 50 mM

sample-2: GaFd 2 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample-2isotropicsample_conditions_1
2D 1H-1H TOCSYsample-2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AZARA, Boucher - processing

ANSIG, Kraulis - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 900 MHz

Related Database Links:

PDB
DBJ BAA10197 BAA24020 BAK50980 BAL29978 BAL33147
GB AAB22616 AAB72025 AGF52490 AIE72625 ALJ68421
PRF 0812212A
REF WP_010873424 WP_028946897
SP P00243 P27320