BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16002

Title: cytoskeletal complex   PubMed: 19074766

Authors: Ithychanda, Sujay Subbayya

Citation: Ithychanda, Sujay Subbayya; Das, Mitali; Ma, Yan-Qing; Ding, Keyang; Wang, Xiaoxia; Gupta, Sudhiranjan; Wu, Chuanyue; Plow, Edward; Qin, Jun. "Migfilin, a molecular switch in regulation of integrin activation"  J. Biol. Chem. 284, 4713-4722 (2009).

Assembly members:
entity_1, polymer, 119 residues, 12434.841 Da.
entity_2, polymer, 24 residues, 2661.174 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GIPEFFQFTVGPLGEGGAHK VRAGGTGLERGVAGVPAEFS IWTREAGAGGLSIAVEGPSK AEIAFEDRKDGSCGVSYVVQ EPGDYEVSIKFNDEHIPDSP FVVPVASLSDDARRLTVTS
entity_2: MASKPEKRVASSVFITLAPP RRDV

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts108
1H chemical shifts748

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 119 residues - 12434.841 Da.

1   GLYILEPROGLUPHEPHEGLNPHETHRVAL
2   GLYPROLEUGLYGLUGLYGLYALAHISLYS
3   VALARGALAGLYGLYTHRGLYLEUGLUARG
4   GLYVALALAGLYVALPROALAGLUPHESER
5   ILETRPTHRARGGLUALAGLYALAGLYGLY
6   LEUSERILEALAVALGLUGLYPROSERLYS
7   ALAGLUILEALAPHEGLUASPARGLYSASP
8   GLYSERCYSGLYVALSERTYRVALVALGLN
9   GLUPROGLYASPTYRGLUVALSERILELYS
10   PHEASNASPGLUHISILEPROASPSERPRO
11   PHEVALVALPROVALALASERLEUSERASP
12   ASPALAARGARGLEUTHRVALTHRSER

Entity 2, entity_2 24 residues - 2661.174 Da.

1   METALASERLYSPROGLULYSARGVALALA
2   SERSERVALPHEILETHRLEUALAPROPRO
3   ARGARGASPVAL

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.1-1 mM; entity_2 0.1-1 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.4; pressure: 1 atm; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1

Software:

X-PLOR, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB55115 BAE32761 BAE41598 BAE41798 BAG51492
EMBL CAA47354
GB AAH04777 AAH19895 AAO15549 AAO49012 AAQ08090
PIR A38203
REF NP_001019386 NP_001019387 NP_001156728 NP_060026 NP_598515
SP Q71FD7 Q8WUP2