BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15988

Title: TonB2 PROTEIN FROM Vibrio anguillarum   PubMed: 18973471

Authors: Lopez, Claudia; Peacock, Sean; Crosa, Jorge; Vogel, Hans

Citation: Lopez, Claudia; Peacock, Sean; Crosa, Jorge; Vogel, Hans. "MOLECULAR CHARACTERIZATION OF THE TonB2 PROTEIN FROM Vibrio anguillarum"  Biochem. J. 418, 49-59 (2009).

Assembly members:
TonB2-CTD, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: Listonella Anguillarum   Taxonomy ID: 55601   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio Anguillarum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TonB2-CTD: AHPFTSAPTFGDFGSNQQAM PLYRVEPVYPSRALKRGVEG FVTLSFTIDTTGKAVDINVV DANPKRMFEREAMQALKKWK YQPQIVDGKAIEQPGQTVTV EFKIAK

Data sets:
Data typeCount
13C chemical shifts279
15N chemical shifts100
1H chemical shifts636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TonB-CTD1

Entities:

Entity 1, TonB-CTD 106 residues - Formula weight is not available

Residues 101-105 are leftover residues of the cleavage product of the expressed protein construct

1   ALAHISPROPHETHRSERALAPROTHRPHE
2   GLYASPPHEGLYSERASNGLNGLNALAMET
3   PROLEUTYRARGVALGLUPROVALTYRPRO
4   SERARGALALEULYSARGGLYVALGLUGLY
5   PHEVALTHRLEUSERPHETHRILEASPTHR
6   THRGLYLYSALAVALASPILEASNVALVAL
7   ASPALAASNPROLYSARGMETPHEGLUARG
8   GLUALAMETGLNALALEULYSLYSTRPLYS
9   TYRGLNPROGLNILEVALASPGLYLYSALA
10   ILEGLUGLNPROGLYGLNTHRVALTHRVAL
11   GLUPHELYSILEALALYS

Samples:

sample_1: TonB2-CTD, [U-100% 13C; U-100% 15N], 0.5 mM; D2O 90%; DSS 1 mM; H2O 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v102.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz

Related Database Links:

GB AAV48777