BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15946

Title: Solution structure of a paralog-specific Mena binder by NMR   PubMed: 19284291

Authors: Link, Nina; Hunke, Cornelia; Eichler, Jutta; Bayer, Peter

Citation: Link, Nina; Hunke, Cornelia; Mueller, Jonathan; Eichler, Jutta; Bayer, Peter. "The solution structure of pGolemi, a high affinity Mena EVH1 binding miniature protein, suggests explanations for paralog-specific binding to Ena/VASP homology (EVH) 1 domains"  Biol. Chem. 390, 417-26 (2009).

Assembly members:
pGolemi, polymer, 30 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
pGolemi: PFPPTPPGEEAPVEDLIRFY NDLQQYLNVV

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pGolemi1

Entities:

Entity 1, pGolemi 30 residues - Formula weight is not available

1   PROPHEPROPROTHRPROPROGLYGLUGLU
2   ALAPROVALGLUASPLEUILEARGPHETYR
3   ASNASPLEUGLNGLNTYRLEUASNVALVAL

Samples:

sample_1: pGolemi 1 mM; H2O 90%; D2O 10%; phosphate buffer 45 mM

sample_conditions_1: ionic strength: 45 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1
NOESYsample_1isotropicsample_conditions_1

Software:

AUREMOL v2.0, Bruker Biospin - chemical shift assignment, peak picking

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB