BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15916

Title: Solution NMR structure of protein ATU0232 from AGROBACTERIUM TUMEFACIENS. Northeast Structural Genomics Consortium (NESG) target AtT3. Ontario Center for Structural Proteomics target ATC0223.

Authors: Lemak, Alexander; Srisailam, Sampath; Yee, Adelinda; Bansal, Sonal; Semesi, Antony; Prestegard, Jim; Arrowsmith, Cheryl

Citation: Lemak, Alexander; Srisailam, Sampath; Yee, Adelinda; Bansal, Sonal; Semesi, Antony; Prestegard, Jim; Arrowsmith, Cheryl. "Solution structure of protein ATC0223 from Agrobacterium Tumefaciens."  Not known ., .-..

Assembly members:
Atc0223, polymer, 83 residues, 6945.835 Da.

Natural source:   Common Name: Agrobacterium tumefaciens   Taxonomy ID: 358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Agrobacterium tumefaciens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Atc0223: MGSSHHHHHHSSGRENLYFQ GMYKFEIYQDKAGEYRFRFK ASNGETMFSSEGYKAKASAI HAIESIKRNSAGADTVDLTT MTA

Data sets:
Data typeCount
13C chemical shifts470
15N chemical shifts117
1H chemical shifts739

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Atc0223_11
2Atc0223_21

Entities:

Entity 1, Atc0223_1 83 residues - 6945.835 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYMETTYRLYSPHEGLUILETYRGLNASP
4   LYSALAGLYGLUTYRARGPHEARGPHELYS
5   ALASERASNGLYGLUTHRMETPHESERSER
6   GLUGLYTYRLYSALALYSALASERALAILE
7   HISALAILEGLUSERILELYSARGASNSER
8   ALAGLYALAASPTHRVALASPLEUTHRTHR
9   METTHRALA

Samples:

sample_1: Atc0223, [U-13C; U-15N], 0.5 mM; TRIS 10 mM; sodium chloride 500 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; Benzamidine 10 mM; H2O 90%; D20 10%

sample_2: Atc0223, [U-13C; U-15N], 0.5 mM; TRIS 10 mM; sodium chloride 500 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; Benzamidine 10 mM; D20 100%

sample_conditions_1: ionic strength: 500 mM; pH: 7.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D (H)C(CO)NH_TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NH_TOCSYsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-13C_arom NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQC(IPAP)sample_1anisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

FMC, Lemak, Steren, Llinas, Arrowsmith - resonance assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAK86048 EGL66960 KEY54649 KJX89794
REF NP_353263 WP_006310075 WP_010970746
SP Q8UIR1