BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15893

Title: Mechanism of metal delivery to the CuA center in terminal oxidases from bacteria: a redox m nage- -trois   PubMed: 18758441

Authors: Bertini, Ivano; Ciofi-Baffoni, Simone; Wang, Shenlin

Citation: Abriata, Luciano; Banci, Lucia; Bertini, Ivano; Ciofi-Baffoni, Simone; Gkazonis, Petros; Spyroulias, Georgios; Vila, Alejandro; Wang, Shenlin. "Mechanism of Cu(A) assembly."  Nat. Chem. Biol. 4, 599-601 (2008).

Assembly members:
CuA center, polymer, 172 residues, 19169.084 Da.

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CuA center: GAMHTFYGTRLLNPKPVDFA LEGPQGPVRLSQFQDKVVLL FFGFTRCPDVCPTTLLALKR AYEKLPPKAQERVQVIFVSV DPERDPPEVADRYAKAFHPS FLGLSGSPEAVREAAQTFGV FYQKSQYRGPGEYLVDHTAT TFVVKEGRLVLLYSPDKAEA TDRVVADLQALL

Data sets:
Data typeCount
13C chemical shifts685
15N chemical shifts163
1H chemical shifts1193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CuA center1

Entities:

Entity 1, CuA center 172 residues - 19169.084 Da.

1   GLYALAMETHISTHRPHETYRGLYTHRARG
2   LEULEUASNPROLYSPROVALASPPHEALA
3   LEUGLUGLYPROGLNGLYPROVALARGLEU
4   SERGLNPHEGLNASPLYSVALVALLEULEU
5   PHEPHEGLYPHETHRARGCYSPROASPVAL
6   CYSPROTHRTHRLEULEUALALEULYSARG
7   ALATYRGLULYSLEUPROPROLYSALAGLN
8   GLUARGVALGLNVALILEPHEVALSERVAL
9   ASPPROGLUARGASPPROPROGLUVALALA
10   ASPARGTYRALALYSALAPHEHISPROSER
11   PHELEUGLYLEUSERGLYSERPROGLUALA
12   VALARGGLUALAALAGLNTHRPHEGLYVAL
13   PHETYRGLNLYSSERGLNTYRARGGLYPRO
14   GLYGLUTYRLEUVALASPHISTHRALATHR
15   THRPHEVALVALLYSGLUGLYARGLEUVAL
16   LEULEUTYRSERPROASPLYSALAGLUALA
17   THRASPARGVALVALALAASPLEUGLNALA
18   LEULEU

Samples:

sample_1: entity, [U-100% 15N], 0.8 ± 0.1 mM; D2O, [U-99.9% 2H], 10%; H2O 90%; Pi 50 mM

sample_2: entity, [U-100% 13C; U-100% 15N], 0.8 ± 0.1 mM; D2O, [U-99.9% 2H], 10%; H2O 90%; Pi 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
15N R2sample_1isotropicsample_conditions_1
15N R1sample_1isotropicsample_conditions_1
1H-15N NOEsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAD71765
GB AAS81921 AEG34333 AFH39882 EIA38408
REF WP_008633914 WP_011173951 WP_011229033 WP_014510964 WP_014630386