BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15880

Title: Structure of Rab11-FIP2 C-terminal Coiled-coil Domain   PubMed: 19119858

Authors: Wei, Jie; Liu, Yuqi; Baleja, James

Citation: Wei, Jie; Liu, Yuqi; Bose, Kakoli; Henry, Gillian; Baleja, Jim. "Disorder and structure in the Rab11 binding domain of Rab11 family interacting protein 2."  Biochemistry 48, 549-557 (2009).

Assembly members:
Rab11-FIP2, polymer, 41 residues, 5004.829 Da.

Natural source:   Common Name: Human   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Human sapien

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Rab11-FIP2: GSLTYEEVLQELVKHKELLR RKDTHIRELEDYIDNLLVRV M

Data sets:
Data typeCount
13C chemical shifts60
15N chemical shifts35
1H chemical shifts299

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21

Entities:

Entity 1, entity_1 41 residues - 5004.829 Da.

1   GLYSERLEUTHRTYRGLUGLUVALLEUGLN
2   GLULEUVALLYSHISLYSGLULEULEUARG
3   ARGLYSASPTHRHISILEARGGLULEUGLU
4   ASPTYRILEASPASNLEULEUVALARGVAL
5   MET

Samples:

sample_1: entity 1 mM; DSS 20 uM; sodium phosphate 20 mM; D2O, [U-100% 2H], 100%

sample_2: entity, [U-100% 15N], 1.5 mM; DSS 20 uM; sodium phosphate 20 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_3: entity, [U-100% 13C; U-100% 15N], 0.8 mM; DSS 20 uM; sodium phosphate 20 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.08 M; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA76785 BAE32923 BAG09909
GB AAH75073 AAH75074 AAH89010 AAI39381 AAI39420
REF NP_001028344 NP_001100917 NP_001157839 NP_001179700 NP_055719
SP G3XA57 Q7L804
TPG DAA14748