BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15876

Title: Human LL-37 Structure   PubMed: 18818205

Authors: Wang, Guangshun

Citation: Wang, Guangshun. "Structures of human host defense cathelicidin LL-37 and its smallest antimicrobial peptide KR-12 in lipid micelles"  J. Biol. Chem. 283, 32637-32643 (2008).

Assembly members:
LL-37, polymer, 37 residues, 4504.398 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LL-37: LLGDFFRKSKEKIGKEFKRI VQRIKDFLRNLVPRTES

Data sets:
Data typeCount
13C chemical shifts106
15N chemical shifts36
1H chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LL-371

Entities:

Entity 1, LL-37 37 residues - 4504.398 Da.

1   LEULEUGLYASPPHEPHEARGLYSSERLYS
2   GLULYSILEGLYLYSGLUPHELYSARGILE
3   VALGLNARGILELYSASPPHELEUARGASN
4   LEUVALPROARGTHRGLUSER

Samples:

sample_1: LL-37, [U-100% 15N], 0.50 mM; LL-37, [U-100% 13C; U-100% 15N], 0.5 mM; LL-37 2 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - peak picking: PIPP, processing: NMR_Pipe

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18114
PDB
DBJ BAJ20821
EMBL CAA61805 CAA86115 CAG33364 CAG46759
GB AAA74084 AAC02634 AAG40802 AAH55089 AAN78318
REF NP_001065283 NP_004336 XP_003818474
SP P49913 Q1KLX1