BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15870

Title: The domain features of the peripheral stalk subunit H of the methanogenic A1AO ATP synthase and the NMR solution structure of H1-47   PubMed: 19580766

Authors: Biukovic, Goran; Gayen, Shovanlal; Pervushin, Konstantin; Gruber, Gerhard

Citation: Biukovic, Goran; Gayen, Shovanlal; Pervushin, Konstantin; Gruber, Gerhard. "Domain features of the peripheral stalk subunit H of the methanogenic A1AO ATP synthase and the NMR solution structure of H(1-47)."  Biophys. J. 97, 286-294 (2009).

Assembly members:
H1-47_subunit_A_ATP_synthase, polymer, 47 residues, 5208.114 Da.

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:   Production method: recombinant technology   Host organism: E. coli

Entity Sequences (FASTA):
H1-47_subunit_A_ATP_synthase: MGVSVMEAIKEVKLAEEQAV KEIEEAKNRAEQIKAEAIEE AKKLIAC

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts49
1H chemical shifts340

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1H1-47_subunit_A_ATP_synthase1

Entities:

Entity 1, H1-47_subunit_A_ATP_synthase 47 residues - 5208.114 Da.

1   METGLYVALSERVALMETGLUALAILELYS
2   GLUVALLYSLEUALAGLUGLUGLNALAVAL
3   LYSGLUILEGLUGLUALALYSASNARGALA
4   GLUGLNILELYSALAGLUALAILEGLUGLU
5   ALALYSLYSLEUILEALACYS

Samples:

sample_1: H1-47 subunit A ATP synthase, [U-99% 13C; U-99% 15N], 0.5 - 1 mM; sodium azide 0.1%; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v1.3, Bruker Biospin - collection, processing

TALOS, Cornilescu, Delaglio and Bax - torsional angle calculation

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAB98215
SP Q57676