BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15843

Title: Solution NMR Structure of the N-Terminal Domain of Protein ECA1580 from Erwinia carotovora, Northeast Structural Genomics Consortium Target EwR156A

Authors: Mills, Jeffrey; Eletsky, Alex; Zhang, Qi; Lee, Dan; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Lui, Jinfeng; Everett, John; Swapna, G. V. T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas

Citation: Mills, Jeffrey; Eletsky, Alex; Zhang, Qi; Lee, Dan; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Lui, Jinfeng; Everett, John; Swapna, G. V. T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas. "NMR Structure of the Putative Cold Shock Protein from Erwinia carotovora: Northeast Structural Genomics Consortium Target EwR156a"  Not known ., .-..

Assembly members:
NC, polymer, 74 residues, 8417.637 Da.

Natural source:   Common Name: Erwinia carotovora   Taxonomy ID: 554   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Erwinia carotovora

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NC: MAMNGTITTWFKDKGFGFIK DENGDNRYFHVIKVANPDLI KKDAAVTFEPTTNNKGLSAY AVKVVPLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts70
1H chemical shifts487

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NC1

Entities:

Entity 1, NC 74 residues - 8417.637 Da.

1   METALAMETASNGLYTHRILETHRTHRTRP
2   PHELYSASPLYSGLYPHEGLYPHEILELYS
3   ASPGLUASNGLYASPASNARGTYRPHEHIS
4   VALILELYSVALALAASNPROASPLEUILE
5   LYSLYSASPALAALAVALTHRPHEGLUPRO
6   THRTHRASNASNLYSGLYLEUSERALATYR
7   ALAVALLYSVALVALPROLEUGLUHISHIS
8   HISHISHISHIS

Samples:

sample_1: NC, [U-100% 13C; U-100% 15N], 1.07 mM; sodium azide 0.02%; DTT 100 mM; calcium chloride 5 mM; sodium chloride 100 mM; MES 20 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_2: NC5, [U-5% 13C; U-100% 15N], 1.864 mM; sodium azide 0.02%; DTT 100 mM; calcium chloride 5 mM; sodium chloride 100 mM; MES 20 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0.235 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
4,3 D GFT HNNACBCAsample_1isotropicsample_conditions_1
4,3 D GFT CABCACONHNsample_1isotropicsample_conditions_1
4,3D HABCABCONHNsample_1isotropicsample_conditions_1
4,3D GFT HCCH COSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D simultaneous NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPSCAN, Glaser - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - structure solution

CSI, (CSI) Wishart and Sykes - structure solution

CYANA v2.1, Guntert, Mumenthaler and Wuthrich, Herrmann, Guntert and Wuthrich - structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis, refinement

PSVS, Bhattacharya and Montelione - refinement

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAH64001 BAS34842 GAJ89345 GAL47698
EMBL CAG74485 CBA21960 CBJ47128 CBX81442 CCC32705
GB ABR77705 ACI09512 ACT12508 ACX88619 ADC57825
REF WP_001251236 WP_002910657 WP_004090082 WP_004102404 WP_004159066