BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15836

Title: Solution NMR structure of protein encoded by MTH693 from Methanobacterium thermoautotrophicum: Northeast Structural Genomics Consortium target tt824a

Authors: Wu, Yibing; Singarapu, Kiran Kumar; Semesi, Anthony; Sukumaran, Dinesh; Yee, Adelinda; Garcia, Maite; Arrowsmith, Cheryl; Szyperski, Thomas

Citation: Wu, Yibing; Singarapu, Kiran Kumar; Semesi, Anthony; Sukumaran, Dinesh; Yee, Adelinda; Garcia, Maite; Arrowsmith, Cheryl; Szyperski, Thomas. "Solution NMR structure of protein encoded by MTH693 from Methanobacterium thermoautotrophicum: Northeast Structural Genomics Consortium target tt824a"  Not known ., .-..

Assembly members:
tt824a, polymer, 101 residues, 10932.419 Da.

Natural source:   Common Name: Methanobacterium thermoautotrophicum   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
tt824a: MGSSHHHHHHSSGRENLYFQ GHMAARITGEPSKKAVSDRL IGRKGVVMEAISPQNSGLVK VDGETWRATSGTVLDVGEEV SVKAIEGVKLVVEKLEEQKG S

Data sets:
Data typeCount
13C chemical shifts374
15N chemical shifts94
1H chemical shifts617

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tt824a1

Entities:

Entity 1, tt824a 101 residues - 10932.419 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETALAALAARGILETHRGLYGLU
4   PROSERLYSLYSALAVALSERASPARGLEU
5   ILEGLYARGLYSGLYVALVALMETGLUALA
6   ILESERPROGLNASNSERGLYLEUVALLYS
7   VALASPGLYGLUTHRTRPARGALATHRSER
8   GLYTHRVALLEUASPVALGLYGLUGLUVAL
9   SERVALLYSALAILEGLUGLYVALLYSLEU
10   VALVALGLULYSLEUGLUGLUGLNLYSGLY
11   SER

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.7 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
4,3D, GFT HNNCABCAsample_1isotropicsample_conditions_1
4,3D, GFT CABCACONNHsample_1isotropicsample_conditions_1
4,3D, GFT HCCH COSYsample_1isotropicsample_conditions_1
3D, 15N-13C RESOLVEDSIMULTANIOUS NOESYsample_1isotropicsample_conditions_1
4,3D, GFT HAHB(CABCA)CONNHsample_1isotropicsample_conditions_1

Software:

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

AutoStruct, Huang, Tejero, Powers and Montelione - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

XEASY, Bartels et al. - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAM69871
GB AAB85198
REF WP_048060880