BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15817

Title: Type III antifreeze protein isoform HPLC-12   PubMed: 19053456

Authors: Siemer, Ansgar; McDermott, Ann

Citation: Siemer, Ansgar; McDermott, Ann. "Solid-State NMR on a Type III Antifreeze Protein in the Presence of Ice"  J. Am. Chem. Soc. 130, 17394-17399 (2008).

Assembly members:
type_III_AFP_isoform_HPLC-12, polymer, 74 residues, Formula weight is not available

Natural source:   Common Name: ocean pout   Taxonomy ID: 8199   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macrozoarces americanus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
type_III_AFP_isoform_HPLC-12: MNQASVVANQLIPINTALTL VMMRSEVVTPVGIPAEDIPR LVSMQVNRAVPLGTTLMPDM VKGYAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts279
15N chemical shifts60
1H chemical shifts352

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 74 residues - Formula weight is not available

1   METASNGLNALASERVALVALALAASNGLN
2   LEUILEPROILEASNTHRALALEUTHRLEU
3   VALMETMETARGSERGLUVALVALTHRPRO
4   VALGLYILEPROALAGLUASPILEPROARG
5   LEUVALSERMETGLNVALASNARGALAVAL
6   PROLEUGLYTHRTHRLEUMETPROASPMET
7   VALLYSGLYTYRALAALALEUGLUHISHIS
8   HISHISHISHIS

Samples:

sample_1: H2O 90%; D2O 10%; sodium phosphate 10 mM; type III AFP isoform HPLC-12, [U-100% 13C; U-100% 15N], 1 mg

sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 297 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

CARA v1.8.4, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UniProt P19614
BMRB 15818
PDB
GB ALL26675
SP P19614