BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15812

Title: NMR Structure of FeoA-like protein from Clostridium acetobutylicum: Northeast Structural Genomics Consortium Target CaR178

Authors: SINGARAPU, KIRAN KUMAR; WU, YIBING; HUA, JIA; SUKUMARAN, DINESH; Zhao, Li; Jiang, Mei; Foote, Erica; Xiao, Rong; Nair, Rajesh; Baran, Michael; G.V.T, Swapna; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; SZYPERSKI, THOMAS

Citation: SINGARAPU, KIRAN KUMAR; WU, YIBING; HUA, JIA; SUKUMARAN, DINESH; Zhao, Li; Jiang, Mei; Foote, Erica; Xiao, Rong; Nair, Rajesh; Baran, Michael; G.V.T, Swapna; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; SZYPERSKI, THOMAS. "NMR Structure of FeoA-like protein from Clostridium acetobutylicum: Northeast Structural Genomics Consortium Target CaR178"  Not known ., .-..

Assembly members:
FeoA-like protein, polymer, 86 residues, 9724.640 Da.

Natural source:   Common Name: Clostridium acetobutylicum   Taxonomy ID: 1488   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium acetobutylicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FeoA-like protein: MTKGIGLNEVEIKSKVKVIG IVPESKVRRKIMDMGIVRGT EIYIEGKAPMGDPIALRLRG YSLSLRKSEAKDILVEVLLE HHHHHH

Data sets:
Data typeCount
13C chemical shifts368
15N chemical shifts78
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FeoA-like protein1

Entities:

Entity 1, FeoA-like protein 86 residues - 9724.640 Da.

1   METTHRLYSGLYILEGLYLEUASNGLUVAL
2   GLUILELYSSERLYSVALLYSVALILEGLY
3   ILEVALPROGLUSERLYSVALARGARGLYS
4   ILEMETASPMETGLYILEVALARGGLYTHR
5   GLUILETYRILEGLUGLYLYSALAPROMET
6   GLYASPPROILEALALEUARGLEUARGGLY
7   TYRSERLEUSERLEUARGLYSSERGLUALA
8   LYSASPILELEUVALGLUVALLEULEUGLU
9   HISHISHISHISHISHIS

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.91 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
4.3D GFT HNNCABCAsample_1isotropicsample_conditions_1
4,3D GFT CABCACONHsample_1isotropicsample_conditions_1
4,3D GFT HABCABCONHsample_1isotropicsample_conditions_1
4,3D GFT HCCH COSYsample_1isotropicsample_conditions_1
3D 15N, 13C resolved NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

AutoStruct, Huang, Tejero, Powers and Montelione - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

Molmol, Koradi, Billeter and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB AAK79005 ADZ20080 AEI31564 KHD35643
REF NP_347665 WP_010964347 WP_034584547