BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15811

Title: Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum

Authors: Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Arrowsmith, Cheryl

Citation: Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Arrowsmith, Cheryl. "Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum/Northeast Structural Genomics Consortium Target TaT88/Ontario Center for Structural Proteomics target ta1093"  Not known ., .-..

Assembly members:
30S_ribosomal_protein_S27A, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Thermoplasma acidophila   Taxonomy ID: 2303   Superkingdom: Archaea   Kingdom: not available   Genus/species: Thermoplasma acidophilum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
30S_ribosomal_protein_S27A: MQKRELYEIADGKLVRKHRF CPRCGPGVFLAEHADRYSCG RCGYTEFKKAKKSKS

Data sets:
Data typeCount
13C chemical shifts224
15N chemical shifts51
1H chemical shifts353

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
130S_ribosomal1
2ZINC ION2

Entities:

Entity 1, 30S_ribosomal 55 residues - Formula weight is not available

1   METGLNLYSARGGLULEUTYRGLUILEALA
2   ASPGLYLYSLEUVALARGLYSHISARGPHE
3   CYSPROARGCYSGLYPROGLYVALPHELEU
4   ALAGLUHISALAASPARGTYRSERCYSGLY
5   ARGCYSGLYTYRTHRGLUPHELYSLYSALA
6   LYSLYSSERLYSSER

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: 30S ribosomal protein S27A, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; ZnSO4 10 uM; DTT, [U-100% 2H], 10 mM; sodium azide 0.01%; benzamidine 10 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: 30S ribosomal protein S27A, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; ZnSO4 10 uM; DTT, [U-100% 2H], 10 mM; sodium azide 0.01%; benzamidine 10 mM; D2O, [U-100% 2H], 100%

sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.95, Goddard - data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAC12221
REF WP_010901503
SP Q9HJ78