BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15742

Title: CsmA   PubMed: 18652828

Authors: Pedersen, Marie; Dittmer, Jens; Underhaug, Jarl; Miller, Mette; Nielsen, Niels

Citation: Pedersen, Marie; Underhaug, Jarl; Dittmer, Jens; Miller, Mette; Nielsen, Niels. "The three-dimensional structure of CsmA: A small antenna protein from the green sulfur bacterium Chlorobium tepidum"  FEBS Lett. 582, 2869-2874 (2008).

Assembly members:
CsmA, polymer, 59 residues, 6160.066 Da.

Natural source:   Common Name: Chlorobium tepidum   Taxonomy ID: 1097   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Chlorobium tepidum

Experimental source:   Production method: purified from the natural source   Host organism: Chlorobium tepidum

Entity Sequences (FASTA):
CsmA: MSGGGVFTDILAAAGRIFEV MVEGHWETVGMLFDSLGKGT MRINRNAYGSMGGGSLRGS

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts369

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CsmA1

Entities:

Entity 1, CsmA 59 residues - 6160.066 Da.

1   METSERGLYGLYGLYVALPHETHRASPILE
2   LEUALAALAALAGLYARGILEPHEGLUVAL
3   METVALGLUGLYHISTRPGLUTHRVALGLY
4   METLEUPHEASPSERLEUGLYLYSGLYTHR
5   METARGILEASNARGASNALATYRGLYSER
6   METGLYGLYGLYSERLEUARGGLYSER

Samples:

sample_1: CsmA, [U-100% 15N], 1.4 mM

sample_conditions_1: ionic strength: 0 M; pH: .; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D NOESY-HSQCsample_1isotropicsample_conditions_1
3D TOCSY-HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker - collection

ARIA v2.2, Linge, O, . - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB AAA18792 AAA18796 AAM73161 ABB23153 ABB27497
PRF 1414247A 1701481A 2115394C 2115394G
REF NP_662819 WP_006366302 WP_010933599 WP_011357028
SP P0A314 P0A315 P15524 P15527