BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15721

Title: Solution structure of protein ATU1203 from AGROBACTERIUM TUMEFACIENS. NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) target ATT10, Ontario Center for Structural Proteomics target ATC1183

Authors: Lemak, Alexander; Gutmanas, Alexandras; Yee, Adelinda; Semesi, Antony; Arrowsmith, Cheryl

Citation: Lemak, Alexander; Gutmanas, Alexandras; Yee, Adelinda; Semesi, Antony; Arrowsmith, Cheryl. "Solution structure of protein Atu1203 from Agrobacterium tumefaciens"  . ., .-..

Assembly members:
ATU1203, polymer, 85 residues, 6335.192 Da.

Natural source:   Common Name: Agrobacterium Tumefaciens   Taxonomy ID: 358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Agrobacterium Tumefaciens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ATU1203: MGSSHHHHHHSSGRENLYFQ GAGLSFHVEDMTCGHCAGVI KGAIEKTVPGAAVHADPASR TVVVGGVSDAAHIAEIITAA GYTPE

Data sets:
Data typeCount
13C chemical shifts221
15N chemical shifts49
1H chemical shifts369

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ATU12031

Entities:

Entity 1, ATU1203 85 residues - 6335.192 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYALAGLYLEUSERPHEHISVALGLUASP
4   METTHRCYSGLYHISCYSALAGLYVALILE
5   LYSGLYALAILEGLULYSTHRVALPROGLY
6   ALAALAVALHISALAASPPROALASERARG
7   THRVALVALVALGLYGLYVALSERASPALA
8   ALAHISILEALAGLUILEILETHRALAALA
9   GLYTYRTHRPROGLU

Samples:

sample_1: ATU1203, [U-13C; U-15N], 0.5 mM; MOPS 10 mM; sodium chloride 450 mM; DTT 10 mM; benzamidine 10 mM; NaN3 0.01%; ZnSO4 10 uM

sample_conditions_1: ionic strength: 450 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C_aliph NOESYsample_1isotropicsample_conditions_1
3D 1H-13C_arom NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

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