BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15674

Title: Solution NMR structure of the chromobox protein homolog 7

Authors: Kaustov, Lilia; Lemak, Alexander; Quyang, Hui; Gutmanas, Alexandras; Fares, Christophe; Bountra, Chas; Weigelt, Johan; Ravichandran, Mani; Loppnau, Peter; Edwards, Aled; Min, Jinrong; Arrowsmith, Cheryl

Citation: Kaustov, Lilia; Lemak, Alexander; Quyang, Hui; Gutmanas, Alexandras; Fares, Christophe; Bountra, Chas; Weigelt, Johan; Ravichandran, Mani; Loppnau, Peter; Edwards, Aled; Min, Jinrong; Arrowsmith, Cheryl. "Solution NMR structure of the chromobox protein homolog 7"  . ., .-..

Assembly members:
cbx7, polymer, 73 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cbx7: MHHHHHHSSGRENLYFQGEQ VFAVESIRKKRVRKGKVEYL VKWKGWPPKYSTWEPEEHIL DPRLVMAYEEKEE

Data typeCount
13C chemical shifts248
15N chemical shifts51
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chromodomain1

Entities:

Entity 1, chromodomain 73 residues - Formula weight is not available

1   METHISHISHISHISHISHISSERSERGLY
2   ARGGLUASNLEUTYRPHEGLNGLYGLUGLN
3   VALPHEALAVALGLUSERILEARGLYSLYS
4   ARGVALARGLYSGLYLYSVALGLUTYRLEU
5   VALLYSTRPLYSGLYTRPPROPROLYSTYR
6   SERTHRTRPGLUPROGLUGLUHISILELEU
7   ASPPROARGLEUVALMETALATYRGLUGLU
8   LYSGLUGLU

Samples:

sample_1: phosphate 10 mM; sodium chloride 300 mM; DTT 2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 300 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D aromat 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 16778 17072 17079
PDB
DBJ BAI46775
GB AAH51773 AAX94038 AAY68391 ABZ92314 AIC56058
REF NP_001179547 NP_001247702 NP_783640 XP_002743799 XP_003126060
SP O95931
TPG DAA29144