BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15665

Title: CONFIRMATIONAL ANALYSIS OF THE BROAD-SPECTRUM ANTIBACTERIAL PEPTIDE, RANTUERIN-2CSA: IDENTIFICATION OF A FULL LENGTH HELIX-TURN-HELIX MOTIF   PubMed: 18387372

Authors: Hewage, Chandralal; Subasinghage, Anusha; Conlon, Michael

Citation: Subasinghage, Anusha; Conlon, J.; Hewage, Chandralal. "Conformational analysis of the broad-spectrum antibacterial peptide, ranatuerin-2CSa: Identification of a full length helix-turn-helix motif."  Biochim. Biophys. Acta 1784, 924-929 (2008).

Assembly members:
ranatuerin-2CSa, polymer, 32 residues, 3256.000 Da.

Natural source:   Common Name: Rana cascadae   Taxonomy ID: 160497   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana cascadae

Experimental source:   Production method: chemical synthesis   Host organism: Rana cascadae

Entity Sequences (FASTA):
ranatuerin-2CSa: GILSSFKGVAKGVAKDLAGK LLETLKCKITGC

Data sets:
Data typeCount
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ranatuerin-2CSa1

Entities:

Entity 1, ranatuerin-2CSa 32 residues - 3256.000 Da.

1   GLYILELEUSERSERPHELYSGLYVALALA
2   LYSGLYVALALALYSASPLEUALAGLYLYS
3   LEULEUGLUTHRLEULYSCYSLYSILETHR
4   GLYCYS

Samples:

Ranatuerin-2CSa: Peptide 1.5-2.0 mM; TFE-d3/H2O 1:1 v/v

Ranatuerin-2CSa_c: pH: 3.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYRanatuerin-2CSaisotropicRanatuerin-2CSa_c
2D 1H-1H NOESYRanatuerin-2CSaisotropicRanatuerin-2CSa_c
2D DQF-COSYRanatuerin-2CSaisotropicRanatuerin-2CSa_c

Software:

xwinnmr v3.5, Bruker Biospin - collection

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - processing

SYBYL v6.8.1, Tripos - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz

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