BMRB Entry 15439

Title:
URN1 FF domain yeast
Deposition date:
2007-08-21
Original release date:
2008-06-26
Authors:
Bonet, Roman; Ramirez-Espain, Ximena; Macias, Maria
Citation:

Citation: Bonet, Roman; Ramirez-Espain, Ximena; Macias, Maria. "Solution structure of the yeast URN1 splicing factor FF domain: Comparative analysis of charge distributions in FF domain structures-FFs and SURPs, two domains with a similar fold"  Proteins 73, 1001-1009 (2008).
PubMed: 18536009

Assembly members:

Assembly members:
FF, polymer, 58 residues, 6868.629 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: petm30

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts397

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 58 residues - 6868.629 Da.

1   ALAMETGLYASPILEASPGLUARGASNILE
2   PHEPHEGLULEUPHEASPARGTYRLYSLEU
3   ASPLYSPHESERTHRTRPSERLEUGLNSER
4   LYSLYSILEGLUASNASPPROASPPHETYR
5   LYSILEARGASPASPTHRVALARGGLUSER
6   LEUPHEGLUGLUTRPCYSGLYGLU

Samples:

sample_1: FF 0.5 mM; sodium phosphate 20 mM; sodium chloride 130 mM; sodium azide 0.03%; D2O 10%; H2O 90%

sample2: FF, [U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 130 mM; sodium azide 0.03%; D2O 10%; H2O 90%

sample3: FF, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 130 mM; sodium azide 0.03%; D2O 10%; H2O 90%

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample2isotropicsample_conditions_1
3D 1H-13C NOESYsample3isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ GAA27107
EMBL CAY87107
GB AAB68289 AAT92759 AFO64407 AFO64408 AFO64409
REF NP_015478
SP Q06525
TPG DAA11564
AlphaFold Q06525