BMRB Entry 11278

Title:
Solution Structure of the CUE Domain in the Human Activating Signal Cointegrator 1 Complex Subunit 2 (ASCC2)
Deposition date:
2010-08-09
Original release date:
2011-08-18
Authors:
Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Yokoyama, S.
Citation:

Citation: Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Yokoyama, S.. "Solution Structure of the CUE Domain in the Human Activating Signal Cointegrator 1 Complex Subunit 2 (ASCC2)"  .

Assembly members:

Assembly members:
CUE domain, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060116-03

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts70
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CUE domain1

Entities:

Entity 1, CUE domain 71 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETCYSGLY
2   VALGLULEUASPSERLEUILESERGLNVAL
3   LYSASPLEULEUPROASPLEUGLYGLUGLY
4   PHEILELEUALACYSLEUGLUTYRTYRHIS
5   TYRASPPROGLUGLNVALILEASNASNILE
6   LEUGLUGLUARGLEUALAPROTHRLEUSER
7   GLNLEUASPARGASNLEUASPARGGLUMET
8   ASN

Samples:

sample_1: CUE domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks