BMRB Entry 11275

Title:
Solution Structure of the RRM Domain in the Human Poly (ADP-ribose) Polymerase Family, Member 10 Variant
Deposition date:
2010-08-09
Original release date:
2011-08-18
Authors:
Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the RRM Domain in the Human Poly (ADP-ribose) Polymerase Family, Member 10 Variant"  .

Assembly members:

Assembly members:
RRM domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050822-12

Data sets:
Data typeCount
13C chemical shifts411
15N chemical shifts92
1H chemical shifts625

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM domain1

Entities:

Entity 1, RRM domain 104 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYVALALA
2   VALGLUVALARGGLYLEUPROPROALAVAL
3   PROASPGLULEULEUTHRLEUTYRPHEGLU
4   ASNARGARGARGSERGLYGLYGLYPROVAL
5   LEUSERTRPGLNARGLEUGLYCYSGLYGLY
6   VALLEUTHRPHEARGGLUPROALAASPALA
7   GLUARGVALLEUALAGLNALAASPHISGLU
8   LEUHISGLYALAGLNLEUSERLEUARGPRO
9   ALAPROPROARGALAPROALAARGLEULEU
10   LEUGLNGLYLEUPROPROGLYTHRSERGLY
11   PROSERSERGLY

Samples:

sample_1: RRM domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks