BMRB Entry 11240

Title:
Solution structure of the e3_binding domain of dihydrolipoamide branched chaintransacylase
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Zhang, H.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Zhang, H.; Hayashi, F.; Yokoyama, S.. "Solution structure of the e3_binding domain of dihydrolipoamide branched chaintransacylase"  .

Assembly members:

Assembly members:
e3_binding domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040510-10

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts274
15N chemical shifts61
1H chemical shifts452

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1e3_binding domain1

Entities:

Entity 1, e3_binding domain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYHISGLNGLU
2   ILELYSGLYARGLYSTHRLEUALATHRPRO
3   ALAVALARGARGLEUALAMETGLUASNASN
4   ILELYSLEUSERGLUVALVALGLYSERGLY
5   LYSASPGLYARGILELEULYSGLUASPILE
6   LEUASNTYRLEUGLULYSGLNTHRGLYALA
7   ILELEUPROPROSERGLYPROSERSERGLY

Samples:

sample_1: e3_binding domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.04, Johnson B.A. - data analysis

Kujira v0.9296, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE90611 BAG64869
GB AAA59200
REF XP_001488662 XP_004471743 XP_005077165 XP_005270602 XP_005270603

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks