BMRB Entry 11225

Title:
Solution structure of the SH3 domain of human SLIT-ROBO Rho GTPase-activating protein 2
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of human SLIT-ROBO Rho GTPase-activating protein 2"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050815-19

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts70
1H chemical shifts458

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 72 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUPROILE
2   GLUALAILEALALYSPHEASPTYRVALGLY
3   ARGTHRALAARGGLULEUSERPHELYSLYS
4   GLYALASERLEULEULEUTYRGLNARGALA
5   SERASPASPTRPTRPGLUGLYARGHISASN
6   GLYILEASPGLYLEUILEPROHISGLNTYR
7   ILEVALVALGLNASPTHRSERGLYPROSER
8   SERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAC52480
REF XP_003410318 XP_004375268 XP_004375269 XP_004610117 XP_004610118

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks