BMRB Entry 11180

Title:
Solution structure of the first SH3 domain of KIAA0318 protein
Deposition date:
2010-07-21
Original release date:
2011-07-20
Authors:
Inoue, K.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first SH3 domain of KIAA0318 protein"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P030512-61

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts100
1H chemical shifts611

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 96 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRSERLYS
2   GLNARGTYRSERGLYLYSVALHISLEUCYS
3   VALALAARGTYRSERTYRASNPROPHEASP
4   GLYPROASNGLUASNPROGLUALAGLULEU
5   PROLEUTHRALAGLYLYSTYRLEUTYRVAL
6   TYRGLYASPMETASPGLUASPGLYPHETYR
7   GLUGLYGLULEULEUASPGLYGLNARGGLY
8   LEUVALPROSERASNPHEVALASPPHEVAL
9   GLNASPASNGLUSERARGLEUALASERTHR
10   SERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.26 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks