BMRB Entry 10315

Title:
Solution structure of the PH domain of Rho GTPase activating protein 21 from human
Deposition date:
2009-03-11
Original release date:
2010-03-11
Authors:
Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of Rho GTPase activating protein 21 from human"  .

Assembly members:

Assembly members:
PH domain, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell-free synthesis   Vector: P051017-10

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts119
1H chemical shifts816

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 125 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPALAALA
2   LYSGLUGLYTRPLEUHISPHEARGPROLEU
3   VALTHRASPLYSGLYLYSARGVALGLYGLY
4   SERILEARGPROTRPLYSGLNMETTYRVAL
5   VALLEUARGGLYHISSERLEUTYRLEUTYR
6   LYSASPLYSARGGLUGLNTHRTHRPROSER
7   GLUGLUGLUGLNPROILESERVALASNALA
8   CYSLEUILEASPILESERTYRSERGLUTHR
9   LYSARGLYSASNVALPHEARGLEUTHRTHR
10   SERASPCYSGLUCYSLEUPHEGLNALAGLU
11   ASPARGASPASPMETLEUALATRPILELYS
12   THRILEGLNGLUSERSERASNLEUASNSER
13   GLYPROSERSERGLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.01 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAA92662 BAG59749
EMBL CAD39153 CAD97787
GB AAM22955 EAW86122 EDL78791 EDL78792 EHH18940
REF NP_001178622 NP_001248214 NP_065875 XP_002806993 XP_003269414
SP Q5T5U3
TPG DAA23594
AlphaFold Q5T5U3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks