BMRB Entry 10137

Title:
Lipocalin-type Prostaglandin D synthase
Deposition date:
2007-05-16
Original release date:
2008-02-20
Authors:
Shimamoto, Shigeru
Citation:

Citation: Shimamoto, Shigeru; Yoshida, T.; Inui, T.; Gohda, K.; Kobayashi, Y.; Fujimori, K.; Tsurumura, T.; Aritake, K.; Urade, Y.; Ohkubo, T.. "NMR solution structure of lipocalin-type prostaglandin D synthase: evidence for partial overlapping of catalytic pocket and retinoic acid-binding pocket within the central cavity."  J. Biol. Chem. 282, 31373-31379 (2007).
PubMed: 17715133

Assembly members:

Assembly members:
L-PGDS, polymer, 167 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pGEX-2T

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts107
1H chemical shifts674

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lipocalin-type Prostaglandin D synthase1

Entities:

Entity 1, Lipocalin-type Prostaglandin D synthase 167 residues - Formula weight is not available

1   GLYSERGLNGLYHISASPTHRVALGLNPRO
2   ASNPHEGLNGLNASPLYSPHELEUGLYARG
3   TRPTYRSERALAGLYLEUALASERASNSER
4   SERTRPPHEARGGLULYSLYSALAVALLEU
5   TYRMETCYSLYSTHRVALVALALAPROSER
6   THRGLUGLYGLYLEUASNLEUTHRSERTHR
7   PHELEUARGLYSASNGLNCYSGLUTHRLYS
8   ILEMETVALLEUGLNPROALAGLYALAPRO
9   GLYHISTYRTHRTYRSERSERPROHISSER
10   GLYSERILEHISSERVALSERVALVALGLU
11   ALAASNTYRASPGLUTYRALALEULEUPHE
12   SERARGGLYTHRLYSGLYPROGLYGLNASP
13   PHEARGMETALATHRLEUTYRSERARGTHR
14   GLNTHRLEULYSASPGLULEULYSGLULYS
15   PHETHRTHRPHESERLYSALAGLNGLYLEU
16   THRGLUGLUASPILEVALPHELEUPROGLN
17   PROASPLYSCYSILEGLNGLU

Samples:

sample_1: Lipocalin-type Prostaglandin D synthase, [U-13C; U-15N], 1.0 ± 0.15 mM

condition_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N HSQCsample_1isotropiccondition_1
2D 1H13C HSQCsample_1isotropiccondition_1
3D HNCOsample_1isotropiccondition_1
3D HNCAsample_1isotropiccondition_1
3D HN(CO)CAsample_1isotropiccondition_1
3D HNCACBsample_1isotropiccondition_1
3D CBCA(CO)NHsample_1isotropiccondition_1
3D HBHA(CBCACO)NHsample_1isotropiccondition_1
3D HCCH-TOCSYsample_1isotropiccondition_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 11062 18473
PDB
DBJ BAA74461 BAE20833
EMBL CAA61506 CAA71226
GB AAH38083 AAH43015 EDL08248 EDL08250 EDL08251
REF NP_032989 XP_006497849 XP_006497850
SP O09114
AlphaFold O09114

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks