BMRB Entry 10073

Title:
C2 domain-containing protein from putative elicitor-responsive gene
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "C2 domain-containing protein from putative elicitor-responsive gene"  .

Assembly members:

Assembly members:
C2 domain, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P031104-14

Data sets:
Data typeCount
13C chemical shifts581
15N chemical shifts134
1H chemical shifts902

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1putative elicitor-responsive gene1

Entities:

Entity 1, putative elicitor-responsive gene 136 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROHISGLY
2   THRLEUGLUVALVALLEUVALSERALALYS
3   GLYLEUGLUASPALAASPPHELEUASNASN
4   METASPPROTYRVALGLNLEUTHRCYSARG
5   THRGLNASPGLNLYSSERASNVALALAGLU
6   GLYMETGLYTHRTHRPROGLUTRPASNGLU
7   THRPHEILEPHETHRVALSERGLUGLYTHR
8   THRGLULEULYSALALYSILEPHEASPLYS
9   ASPVALGLYTHRGLUASPASPALAVALGLY
10   GLUALATHRILEPROLEUGLUPROVALPHE
11   VALGLUGLYSERILEPROPROTHRALATYR
12   ASNVALVALLYSASPGLUGLUTYRLYSGLY
13   GLUILETRPVALALALEUSERPHELYSPRO
14   SERGLYPROSERSERGLY

Samples:

sample_1: C2 domain, [U-13C; U-15N], 1.19 mM; NaPi 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAG52148 AAK96814 AAM10066 AAM63058 AEE34071
REF NP_176511 XP_002887967
SP Q9C8S6
AlphaFold Q9C8S6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks