BMRB Entry 10068

Title:
Solution Structure of the CH domain from Mouse Trangelin
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the CH domain from Mouse Trangelin"  .

Assembly members:

Assembly members:
Calponin homology (CH) domain, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P020520-56

Data sets:
Data typeCount
13C chemical shifts609
15N chemical shifts146
1H chemical shifts989

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Transgelin1

Entities:

Entity 1, Transgelin 144 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUGLULEU
2   GLUGLUARGLEUVALGLUTRPILEVALVAL
3   GLNCYSGLYPROASPVALGLYARGPROASP
4   ARGGLYARGLEUGLYPHEGLNVALTRPLEU
5   LYSASNGLYVALILELEUSERLYSLEUVAL
6   ASNSERLEUTYRPROGLUGLYSERLYSPRO
7   VALLYSVALPROGLUASNPROPROSERMET
8   VALPHELYSGLNMETGLUGLNVALALAGLN
9   PHELEULYSALAALAGLUASPTYRGLYVAL
10   ILELYSTHRASPMETPHEGLNTHRVALASP
11   LEUTYRGLUGLYLYSASPMETALAALAVAL
12   GLNARGTHRLEUMETALALEUGLYSERLEU
13   ALAVALTHRLYSASNASPGLYASNTYRARG
14   GLYASPPROASNTRPPHEMETLYSSERGLY
15   PROSERSERGLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.0 mM; Tris-HCl(pH 7.0) 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.820, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA21811 BAA21839 BAB22427 BAC36700 BAG36752
EMBL CAA92941 CAG46482 CAH18406
GB AAA58375 AAA79165 AAA79166 AAC08010 AAC21582
REF NP_001001522 NP_003177 NP_035656 XP_003253239 XP_003253241
SP P37804 Q01995
AlphaFold P37804 Q01995

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks