BMRB Entry 10024

Title:
Chemical shift assignments for the Rhodobacter sphaeroides PufX membrane protein
Deposition date:
2006-08-03
Original release date:
2007-06-13
Authors:
Wang, Zheng-Yu; Suzuki, H.; Kudo, M.; Kobayashi, M.; Nozawa, T.
Citation:

Citation: Wang, Zheng-Yu; Suzuki, H.; Kobayashi, M.; Nozawa, T.. "Solution structure of the Rhodobacter sphaeroides PufX membrane protein: implications for the quinone exchange and protein-protein interactions."  Biochemistry 46, 3635-3642 (2007).
PubMed: 17335288

Assembly members:

Assembly members:
PufX polypeptide, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rhodobacter sphaeroides   Taxonomy ID: 1063   Superkingdom: bacteria   Kingdom: Not applicable   Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts133
15N chemical shifts74
1H chemical shifts352

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 77 residues - Formula weight is not available

1   ALAASPLYSTHRILEPHEASNASPHISLEU
2   ASNTHRASNPROLYSTHRASNLEUARGLEU
3   TRPVALALAPHEGLNMETMETLYSGLYALA
4   GLYTRPALAGLYGLYVALPHEPHEGLYTHR
5   LEULEULEUILEGLYPHEPHEARGVALVAL
6   GLYARGMETLEUPROILEGLNGLUASNGLN
7   ALAPROALAPROASNILETHRGLYALALEU
8   GLUHISHISHISHISHISHIS

Samples:

sample_1: PufX polypeptide, [U-13C; U-15N], 2 mM; Chloroform 50%; Methanol 50%

condition_1: temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQC-TOCSYsample_1not availablecondition_1
HSQC-NOESYsample_1not availablecondition_1
HNCAsample_1not availablecondition_1
HN(CO)CAsample_1not availablecondition_1
CBCANHsample_1not availablecondition_1
CBCA(CO)NHsample_1not availablecondition_1
HNHAsample_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance DRX400 400 MHz

Related Database Links:

PDB
EMBL CAB38756
GB AAA26129 AAF24306 ABA79427 ABN77002 ACM01440
REF WP_002720419 YP_353328
SP P13402
AlphaFold P13402

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks