BMRB Entry 7324

Title:
NMR solution structure of mouse SelW
Deposition date:
2006-10-26
Original release date:
2006-11-06
Authors:
Aachmann, Finn; Fomenko, Dmitri; Soragni, Alice; Gladyshev, Vadim; Dikiy, Alexander
Citation:

Citation: Aachmann, Finn; Fomenko, Dmitri; Soragni, Alice; Gladyshev, Vadim; Dikiy, Alexander. "Solution structure of selenoprotein W and NMR analysis of its interaction with 14-3-3 proteins."  J. Biol. Chem. 282, 37036-37044 (2007).
PubMed: 17928294

Assembly members:

Assembly members:
SelW, polymer, 96 residues, 10463 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pFA6

Data sets:
Data typeCount
13C chemical shifts360
15N chemical shifts95
1H chemical shifts639

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SelW1

Entities:

Entity 1, SelW 96 residues - 10463 Da.

SELENOCYSTEINE (CSE) HAS BEEN MUTATED TO CYS. Amino acid 89-96 His-tag, cloning artifact.

1   METALALEUALAVALARGVALVALTYRSER
2   GLYALACYSGLYTYRLYSPROLYSTYRLEU
3   GLNLEULYSGLULYSLEUGLUHISGLUPHE
4   PROGLYCYSLEUASPILECYSGLYGLUGLY
5   THRPROGLNVALTHRGLYPHEPHEGLUVAL
6   THRVALALAGLYLYSLEUVALHISSERLYS
7   LYSARGGLYASPGLYTYRVALASPTHRGLU
8   SERLYSPHEARGLYSLEUVALTHRALAILE
9   LYSALAALALEUALAGLNCYSGLNLEUGLU
10   HISHISHISHISHISHIS

Samples:

sample_1: SelW, [U-98% 13C; U-99% 15N], 1 ± 0.1 mM; phosphate buffer 25 mM; NaCl 50 mM; DTT 2.5 mM; H2O 90%; D2O 10%

conditions_1: pH: 5.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H13C_HSQCsample_1not availableconditions_1
2D_1H_NOESYsample_1not availableconditions_1
2D_1H_TOCSYsample_1not availableconditions_1
2D_1H_IP-COSYsample_1not availableconditions_1
3D_15N-separated_NOESYsample_1not availableconditions_1
3D_13C-separated_NOESYsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
CACBNHsample_1not availableconditions_1
CACB(CO)NHsample_1not availableconditions_1
HAHBNHsample_1not availableconditions_1
HAHB(CO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1

Software:

CARA v1.4 - data analysis

xwinnmr v3.5 - collection

NMR spectrometers:

  • Bruker Avence AV 600 MHz

Related Database Links:

PDB
DBJ BAC55247 BAC55256
GB AAB69860 AAC52255 AAC53317 AAF64481 AAH52719
REF NP_033182 NP_037159 XP_005139276 XP_005372482
SP P63300 P63301
AlphaFold P63300 P63301

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks