BMRB Entry 7313

Title:
1H, 13C and 15N Backbone Resonance Assignments of the Pactolus I domain
Deposition date:
2006-10-09
Original release date:
2007-06-20
Authors:
Sen, Mehmet; Legge, Glen
Citation:

Citation: Sen, Mehmet; Legge, Glen. "Pactolus I-domain: Functional switching of the Rossmann fold"  Proteins 68, 626-635 (2007).
PubMed: 17523188

Assembly members:

Assembly members:
Pactolus I-domain, polymer, 230 residues, 25366 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts161
1H chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1I-domain1

Entities:

Entity 1, I-domain 230 residues - 25366 Da.

This sequence contains hexameric Histidine Tag

1   GLYSERSERHISHISHISHISHISHISGLY
2   LEUVALPROARGGLYSERHISASPASNSER
3   VALASPLEUTYRPHELEUMETGLYLEUSER
4   GLYSERALAGLNGLYHISLEUSERASNVAL
5   GLNTHRLEUGLYSERASPLEULEULYSALA
6   LEUASNGLUILESERARGSERGLYARGILE
7   GLYPHEGLYSERILEVALASNMETTHRPHE
8   GLNHISILELEULYSLEUTHRALAASPSER
9   SERGLNPHEGLNARGGLULEUARGLYSGLN
10   LEUVALSERGLYLYSLEUALATHRPROLYS
11   GLYGLNLEUASPALAVALVALGLNVALALA
12   ILECYSLEUGLYGLUILEGLYTRPARGASN
13   GLYTHRARGPHELEUVALLEUVALTHRASP
14   ASNASPPHEHISLEUALALYSASPLYSTHR
15   LEUGLYTHRARGGLNASNTHRSERASPGLY
16   ARGCYSHISLEUASPASPGLYMETTYRARG
17   SERARGGLYGLUPROASPTYRGLNSERVAL
18   VALGLNLEUALASERLYSLEUALAGLUASN
19   ASNILEGLNPROILEPHEVALVALPROSER
20   ARGMETVALLYSTHRTYRGLULYSLEUTHR
21   THRPHEILEPROLYSLEUTHRILEGLYGLU
22   LEUSERASPASPSERSERASNVALALAGLN
23   LEUILEARGASNALATYRSERLYSLEUSER

Samples:

triple_labelled_sample: Pactolus I-domain, [U-99% 2H; U-99% 15N; U-99% 13C], 0.3 ± 0.01 mM; dTris 10 mM; NaN3 0.05%; D20 5%

double_labelled_sample: Pactolus I-domain, [U-99% 15N; U-99% 13C], 0.3 ± 0.01 mM; dTris 10 mM; NaN3 0.05%; D20 5%

15N_labelled_sample: Pactolus I-domain, [U-99% 15N], 0.3 ± 0.01 mM; dTris 10 mM; NaN3 0.05%; D20 5%

conditions: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAtriple_labelled_samplenot availableconditions
HN(CO)CAtriple_labelled_samplenot availableconditions
HNCBtriple_labelled_samplenot availableconditions
HN(CO)CBnot availablenot availableconditions
HN(CA)COtriple_labelled_samplenot availableconditions
HNCOtriple_labelled_samplenot availableconditions
HSQC-NOESY-HSQCtriple_labelled_samplenot availableconditions
CACB(CO)NHtriple_labelled_samplenot availableconditions
CN-NOESYtriple_labelled_samplenot availableconditions
N-TOCSY15N_labelled_samplenot availableconditions
N-NOESY15N_labelled_samplenot availableconditions
HSQC15N_labelled_samplenot availableconditions

Software:

NMRPipe - Data Processing

NMRView - Data Analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAE23399
GB AAC25502 AAI13143 EDL03670 EDL03671
REF NP_032431 XP_006522988 XP_006522989 XP_006522990 XP_006522991
SP Q3UV74
AlphaFold Q3UV74

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks