BMRB Entry 7303

Title:
1H, 13C and 15N resonance assignments for proapoptotic protein Nix (1~156) from Danio rerio
Deposition date:
2006-09-26
Original release date:
2007-06-26
Authors:
Chu, Feng-I; Ho, Yu; Hung, Chang-Jen; Tzou, Der-Lii, M
Citation:

Citation: Chu, Feng-I; Ho, Yu; Huang, Chang-Jen; Tzou, Der-Lii. "1H, 13C and 15N resonance assignments for proapoptotic protein Nix (residue 1 approximately 156) from Danio rerio"  Biomol. NMR Assignments 1, 29-31 (2007).
PubMed: 19636819

Assembly members:

Assembly members:
15N Zebrafish NIX, polymer, 164 residues, 18039 Da.

Natural source:

Natural source:   Common Name: Zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts512
15N chemical shifts129
1H chemical shifts761

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NIX1

Entities:

Entity 1, NIX 164 residues - 18039 Da.

1   METSERTHRTHRALAALAALAGLNLEUASN
2   ASNASNGLUGLUPROGLYLEUASNGLYSER
3   TRPVALGLULEUGLUMETASNGLYASNTHR
4   GLNHISGLNSERPROSERLEUPROSERLEU
5   LEUGLYASNGLYASNGLNGLYGLUPROGLN
6   ALASERGLNALALEUGLUALAVALGLNGLU
7   GLUGLUGLUVALLEUTHRGLYGLYLEUGLU
8   HISVALPROSERSERSERSERILEHISASN
9   GLYASPMETGLULYSILELEULEUASPALA
10   GLNHISGLUSERSERPROSERSERSERSER
11   CYSASNSERPROPROARGPROHISSERPRO
12   ASPGLNASPGLUGLYGLNILEMETPHEASP
13   VALGLUMETPROSERGLUARGASPCYSGLN
14   SERGLUASPASPSERMETGLULYSASPLYS
15   GLUASPASPILELEUMETASNLYSGLYGLU
16   SERTRPVALALAASPTRPPHEGLUHISHIS
17   HISHISHISHIS

Samples:

Zebrafish_NIX_1: Zebrafish NIX, [U-90% 15N], 1.5 ± 0.1 mM

Zebrafish_NIX_2: Zebrafish NIX, [U-95% 13C; U-90% 15N], 1.5 ± 0.1 mM

pH_6.5_PBS_buffer: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCZebrafish_NIX_1not availablepH_6.5_PBS_buffer
HNCOZebrafish_NIX_2not availablepH_6.5_PBS_buffer
HN(CA)COZebrafish_NIX_2not availablepH_6.5_PBS_buffer
HNCAZebrafish_NIX_2not availablepH_6.5_PBS_buffer
HN(CO)CAZebrafish_NIX_2not availablepH_6.5_PBS_buffer
CBCANHZebrafish_NIX_2not availablepH_6.5_PBS_buffer
CBCA(CO)NHZebrafish_NIX_2not availablepH_6.5_PBS_buffer
HCCCONH_CZebrafish_NIX_2not availablepH_6.5_PBS_buffer
HCCCONH_HZebrafish_NIX_2not availablepH_6.5_PBS_buffer
HBHA(CO)NHZebrafish_NIX_2not availablepH_6.5_PBS_buffer
TOSCY-HSQCZebrafish_NIX_2not availablepH_6.5_PBS_buffer

Software:

xwinnmr v3.0.b.13 - data processing

AURELIA v2.5.7 - data analysis

NMR spectrometers:

  • BRUKER AVANCE 600 MHz

Related Database Links:

GB AAI63834 AAI63857 AAR83677
REF NP_001012242

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks