BMRB Entry 7223

Title:
Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics target HR2873B
Deposition date:
2006-07-14
Original release date:
2009-05-28
Authors:
Aramini, J.; Ho, C.; Yin, C.; Cunningham, K.; Janjua, H.; Ma, L.-C.; Xiao, R.; Acton, T.; Montelione, G.
Citation:

Citation: Aramini, J.; Ho, C.; Yin, C.; Cunningham, K.; Janjua, H.; Li, M.-C.; Xiao, R.; Acton, T.; Montelione, G.. "Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics target HR2873B."  .

Assembly members:

Assembly members:
Interferon-induced 17 kDa protein, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts86
1H chemical shifts605
coupling constants57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Interferon-induced 17 kDa protein1

Entities:

Entity 1, Interferon-induced 17 kDa protein 88 residues - Formula weight is not available

1   METASPGLUPROLEUSERILELEUVALARG
2   ASNASNLYSGLYARGSERSERTHRTYRGLU
3   VALARGLEUTHRGLNTHRVALALAHISLEU
4   LYSGLNGLNVALSERGLYLEUGLUGLYVAL
5   GLNASPASPLEUPHETRPLEUTHRPHEGLU
6   GLYLYSPROLEUGLUASPGLNLEUPROLEU
7   GLYGLUTYRGLYLEULYSPROLEUSERTHR
8   VALPHEMETASNLEUARGLEUARGGLYGLY
9   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Interferon-induced 17 kDa protein, [U-13C; U-15N], 0.81 mM; ammonium citrate 50 mM; CaCl2 5 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_2: Interferon-induced 17 kDa protein, [U-5% 13C; U-15N], 0.9 mM; ammonium citrate 50 mM; CaCl2 5 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
high resolution 2D CH-HSQC (for stereospecific assignment of Val/Leu methyls)not availablenot availablenot available
2D 15N 1H heteronuclear NOEnot availablenot availablenot available
3D TR backbone exptsnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D HCCH-TOCSYsnot availablenot availablenot available

Software:

xwinnmr v3.5pl6 - collection

VNMR v6.1C - collection

AutoAssign v2.2.1 - data analysis

SPARKY v3.110 - data analysis

AutoStruct v2.1.1 - refinement

X-PLOR NIH v2.11.2 - refinement

NMRPipe v2.3 - processing

PDBStat v4.1 - data analysis

PSVS v1.3 - data analysis

CNS v1.1 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 5658
PDB
DBJ BAJ20347
GB AAA36038 AAA36128 AAH09507 AAN86983 AAP35961
REF NP_005092 XP_003806031 XP_004024480 XP_009452953 XP_520842
SP P05161
AlphaFold P05161

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks