BMRB Entry 7165

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Saccharomyces cerevisiae Urm1
Deposition date:
2006-06-10
Original release date:
2006-08-11
Authors:
Xu, Junjie; Zhang, Jiahai; Huang, Hongda; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Xu, Junjie; Zhang, Jiahai; Wang, Li; Zhou, Jie; Huang, Hongda; Wu, Jihui; Zhong, Yang; Shi, Yunyu. "Solution structure of Urm1 and its implications for the origin of protein modifiers"  Proc. Natl. Acad. Sci. U.S.A. 103, 11625-11630 (2006).
PubMed: 16864801

Assembly members:

Assembly members:
Urm1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts102
1H chemical shifts688

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Urm11

Entities:

Entity 1, Urm1 107 residues - Formula weight is not available

1   METVALASNVALLYSVALGLUPHELEUGLY
2   GLYLEUASPALAILEPHEGLYLYSGLNARG
3   VALHISLYSILELYSMETASPLYSGLUASP
4   PROVALTHRVALGLYASPLEUILEASPHIS
5   ILEVALSERTHRMETILEASNASNPROASN
6   ASPVALSERILEPHEILEGLUASPASPSER
7   ILEARGPROGLYILEILETHRLEUILEASN
8   ASPTHRASPTRPGLULEUGLUGLYGLULYS
9   ASPTYRILELEUGLUASPGLYASPILEILE
10   SERPHETHRSERTHRLEUHISGLYGLYLEU
11   GLUHISHISHISHISHISHIS

Samples:

sample_1: Urm1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 10%

sample_2: Urm1, [U-13C; U-15N], 0.8 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 10%

sample_3: Urm1, [U-13C; U-15N], 0.8 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 99.96%

conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
CBCACONHsample_2not availableconditions_1
CBCANHsample_2not availableconditions_1
HNCOsample_2not availableconditions_1
HN(CA)COsample_2not availableconditions_1
C(CO)NH-TOCSYsample_2not availableconditions_1
H(CCO)NH-TOCSYsample_2not availableconditions_1
15N TOCSYsample_2not availableconditions_1
HBHA(CBCACO)NHsample_2not availableconditions_1
15N NOESYsample_2not availableconditions_1
13C NOESYsample_2not availableconditions_1
HCCH-TOCSYsample_3not availableconditions_1
HCCH-COSYsample_3not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ GAA24107
EMBL CAA86243 CAY80504
GB AAS56621 AHY75989 EDN61485 EDV09498 EEU09048
REF NP_012258
SP A6ZVR0 B3LTL7 P40554
TPG DAA08538
AlphaFold P40554 A6ZVR0 B3LTL7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks