BMRB Entry 7162

Title:
1H and 15N assignments for the b' domain of ERp57
Deposition date:
2006-06-09
Original release date:
2006-10-30
Authors:
Kozlov, Guennadi; Maattanen, Pekka; Schrag, Joseph; Pollock, Stephanie; Cygler, Miroslaw; Nagar, Bhushan; Thomas, David; Gehring, Kalle
Citation:

Citation: Kozlov, Guennadi; Maattanen, Pekka; Schrag, Joseph; Pollock, Stephanie; Cygler, Miroslaw; Nagar, Bhushan; Thomas, David; Gehring, Kalle. "Crystal structure of the bb' domains of the protein disulfide isomerase, ERp57"  Structure 14, 1331-1339 (2006).
PubMed: 16905107

Assembly members:

Assembly members:
b' domain, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
15N chemical shifts123
1H chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1b' domain of ERp571

Entities:

Entity 1, b' domain of ERp57 145 residues - Formula weight is not available

1   GLYPROLEUGLYSERPHEGLYILECYSPRO
2   HISMETTHRGLUASPASNLYSASPLEUILE
3   GLNGLYLYSASPLEULEUILEALATYRTYR
4   ASPVALASPTYRGLULYSASNALALYSGLY
5   SERASNTYRTRPARGASNARGVALMETMET
6   VALALALYSLYSPHELEUASPALAGLYHIS
7   LYSLEUASNPHEALAVALALASERARGLYS
8   THRPHESERHISGLULEUSERASPPHEGLY
9   LEUGLUSERTHRALAGLYGLUILEPROVAL
10   VALALAILEARGTHRALALYSGLYGLULYS
11   PHEVALMETGLNGLUGLUPHESERARGASP
12   GLYLYSALALEUGLUARGPHELEUGLNASP
13   TYRPHEASPGLYASNLEULYSARGTYRLEU
14   LYSSERGLUPROILEPROGLUSERASNASP
15   GLYALAALAALASER

Samples:

sample_1: b' domain 1 mM; phosphate buffer 50 mM; NaCl 100 mM; DTT 1 mM

sample_2: b' domain, [U-15N], 1 mM; phosphate buffer 50 mM; NaCl 100 mM; DTT 1 mM

sample_3: b' domain, [U-13C; U-15N], 1 mM; phosphate buffer 50 mM; NaCl 100 mM; DTT 1 mM

conditions_1: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_3not availableconditions_1
CBCA(CO)NHsample_3not availableconditions_1
15N-NOESYsample_2not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

BMRB 17224
PDB
DBJ BAA03759 BAA11928 BAG52144 BAG52151 BAG56782
EMBL CAA89996 CAH90193
GB AAB37397 AAC50331 AAC51518 AAH14433 AAH36000
PRF 2201310A 2209333A
REF NP_001127250 NP_001162437 NP_001164786 NP_001182041 NP_001182645
SP P30101 Q4VIT4 Q5RDG4
AlphaFold Q4VIT4 P30101 Q5RDG4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks