BMRB Entry 7131

Title:
Solution structure of IGF2R domain 11
Deposition date:
2006-05-24
Original release date:
2008-08-13
Authors:
Williams, Christopher; Crump, Matthew
Citation:

Citation: Williams, Christopher; Rezgui, Dellel; Prince, Stuart; Zaccheo, Oliver; Foulstone, Emily; Forbes, Briony; Norton, Raymond; Crosby, John; Hassan, A.; Crump, Matthew. "Structural insights into the interaction of insulin-like growth factor 2 with IGF2R domain 11."  Structure 15, 1065-1078 (2007).
PubMed: 17850746

Assembly members:

Assembly members:
domain 11, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts595
15N chemical shifts147
1H chemical shifts899

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1domian 111

Entities:

Entity 1, domian 11 151 residues - Formula weight is not available

C-terminal His tag from vector

1   METLYSSERASNGLUHISASPASPCYSGLN
2   VALTHRASNPROSERTHRGLYHISLEUPHE
3   ASPLEUSERSERLEUSERGLYARGALAGLY
4   PHETHRALAALATYRSERGLULYSGLYLEU
5   VALTYRMETSERILECYSGLYGLUASNGLU
6   ASNCYSPROPROGLYVALGLYALACYSPHE
7   GLYGLNTHRARGILESERVALGLYLYSALA
8   ASNLYSARGLEUARGTYRVALASPGLNVAL
9   LEUGLNLEUVALTYRLYSASPGLYSERPRO
10   CYSPROSERLYSSERGLYLEUSERTYRLYS
11   SERVALILESERPHEVALCYSARGPROGLU
12   ALAGLYPROTHRASNARGPROMETLEUILE
13   SERLEUASPLYSGLNTHRCYSTHRLEUPHE
14   PHESERTRPHISTHRPROLEUALACYSGLU
15   GLNALATHRGLULYSHISHISHISHISHIS
16   HIS

Samples:

sample_1: domain 11, [U-95% 13C; U-95% 15N], 1 ± 0.1 mM; NaOAc 20 mM; EDTA 1 mM; benzamidine 1 mM; NaN3 100 uM; NaN3 5%

sample_2: domain 11, [U-95% 15N], 1 ± 0.1 mM; NaOAc 20 mM; EDTA 1 mM; benzamidine 1 mM; NaN3 100 uM; NaN3 5%

sample_3: domain 11, [U-99% 12C; U-99% 14N], 1 ± 0.1 mM; NaOAc 20 mM; EDTA 1 mM; benzamidine 1 mM; NaN3 100 uM; NaN3 5%

conditions_1: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNCACOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCOCAnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1
HCCH-TCOSYnot availablenot availableconditions_1
15N HSQC-NOESYnot availablenot availableconditions_1
15N HSQC-TOCSYnot availablenot availableconditions_1
1H-1H NOESYnot availablenot availableconditions_1
1H-1H TOCSYnot availablenot availableconditions_1
13C/15N NOESYnot availablenot availableconditions_1

Software:

CNS v1.2 - structure calculation

NMRPipe_NMRDraw - data processing

SPARKY v3.12 - assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17127 17128
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks