BMRB Entry 7085

Title:
Assignment of hypothetical protein yst6499
Deposition date:
2006-04-24
Original release date:
2006-10-20
Authors:
Wu, Bin; Yee, Adelina; Lemak, Alexander; Ramelot, Theresa; Semesi, Anthony; Kennedy, Michael; Arrowsmith, Cheryl
Citation:

Citation: Wu, Bin; Yee, Adelina; Lemak, Alexander; Ramelot, Theresa; Semesi, Anthony; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of hypothetical protein yst6499 from Saccharomyces cerevisiae/ Northeast Structural Genomics Consortium Target YT727/ Ontario Center for Structural Proteomics Target yst6499"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
hypothetical protein yst6499, polymer, 120 residues, 13343.00 Da.

Natural source:

Natural source:   Common Name: Saccharomyces cerevisiae   Taxonomy ID: 2303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET15B

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts91
1H chemical shifts679

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yst64991

Entities:

Entity 1, yst6499 120 residues - 13343.00 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETLYSSERSERILEPROILETHR
4   GLUVALLEUPROARGALAVALGLYSERLEU
5   THRPHEASPGLUASNTYRASNLEULEUASP
6   THRSERGLYVALALALYSVALILEGLULYS
7   SERPROILEALAGLUILEILEARGLYSSER
8   ASNALAGLULEUGLYARGLEUGLYTYRSER
9   VALTYRGLUASPALAGLNTYRILEGLYHIS
10   ALAPHELYSLYSALAGLYHISPHEILEVAL
11   TYRPHETHRPROLYSASNLYSASNARGGLU
12   GLYVALVALPROPROVALGLYILETHRASN

Samples:

sample_1: hypothetical protein yst6499, [U-13C; U-15N], 1 mM; NaCl 250 mM; tris 10 mM

cond_set1: ionic strength: 0.25 M; pH: 7.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCAcoNHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNcoCAnot availablenot availablenot available
CCTOCSY_NHnot availablenot availablenot available
HCCTOCSY_NHnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HCCHTOCSYnot availablenot availablenot available
HCCHCOSYnot availablenot availablenot available
CCHTOCSYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
13C NOESYnot availablenot availablenot available

Software:

SPARKY v3.95 - assignemt, spectral analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ GAA26128
EMBL CAY82229
GB AHY77122 EDN62841 EDV12232 EEU08283 EIW08126
REF NP_061494
SP Q3E841
TPG DAA10576
AlphaFold Q3E841

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks