BMRB Entry 7074

Title:
1H, 13C and 15N chemical shift assignments of the protein Pf0610 from pyrococcus furiosus
Deposition date:
2006-04-18
Original release date:
2006-06-28
Authors:
Wang, Xu; Lee, Hanseung; Prestegard, James
Citation:

Citation: Wang, Xu; Lee, Han-Seung; Sugar, Frank; Jenney, Francis; Adams, Michael; Prestegard, James. "PF0610, a novel winged helix-turn-helix variant possessing a rubredoxin-like Zn ribbon motif from the hyperthermophilic archaeon, Pyrococcus furiosus"  Biochemistry 46, 752-761 (2007).
PubMed: 17223696

Assembly members:

Assembly members:
Pf0610, polymer, 105 residues, 12139 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts90
1H chemical shifts660
residual dipolar couplings209

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pf06101
2ZINC (II) ION2

Entities:

Entity 1, Pf0610 105 residues - 12139 Da.

The first 9 residues are part of the His tag. Pf0610 protein starts at residue 10

1   ALAHISHISHISHISHISHISGLYSERALA
2   THRARGARGGLULYSILEILEGLULEULEU
3   LEUGLUGLYASPTYRSERPROSERGLULEU
4   ALAARGILELEUASPMETARGGLYLYSGLY
5   SERLYSLYSVALILELEUGLUASPLEULYS
6   VALILESERLYSILEALALYSARGGLUGLY
7   METVALLEULEUILELYSPROALAGLNCYS
8   ARGLYSCYSGLYPHEVALPHELYSALAGLU
9   ILEASNILEPROSERARGCYSPROLYSCYS
10   LYSSERGLUTRPILEGLUGLUPROARGPHE
11   LYSLEUGLUARGLYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Pf0610, [U-13C; U-15N], 0.5 ± 0.1 mM; Tris 20 ± 2 mM; NaCl 150 ± 20 mM; D2O 5 ± 1 %

sample_2: Pf0610, [U-13C; U-15N], 0.5 ± 0.1 mM; Tris 20 ± 2 mM; NaCl 150 ± 20 mM; D2O 5 ± 1 %; Pentaethylene glycol monodecyl ether 8 ± 1 %; hexanol 1.5 ± 0.5 %

conditions_1: pH: 8.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-edited NOESY-HSQCnot availablenot availableconditions_1
13C-edited NOESY-HSQCnot availablenot availableconditions_1
aromatic 13C-edited NOESY-HSQCnot availablenot availableconditions_1

Software:

NMRView v5.2.2, One Moon Scientific - Analysis

NMRPipe v2.2, NIH - Analysis

NMR spectrometers:

  • Varian Unity 600 600 MHz
  • Varian Unity 800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks