BMRB Entry 6936

Title:
Resonance assignments for the pKM101 homologue of VirB7 (TraN) in complex with the pKM101 homologue of VirB9 (TraO)
Deposition date:
2005-01-13
Original release date:
2006-04-14
Authors:
Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul
Citation:

Citation: Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul. "Resonance Assignments of the Complex between TraN and the C-terminal Domain of TraO from the Conjugative Plasmid pKM101"  J. Biomol. NMR 36, 31-31 (2006).
PubMed: 16601857

Assembly members:

Assembly members:
VirB7, polymer, 36 residues, 3772 Da.
VirB9, polymer, 126 residues, 14060 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts634
15N chemical shifts151
1H chemical shifts1016

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VirB71
2VirB92

Entities:

Entity 1, VirB7 36 residues - 3772 Da.

GAMA is part of the tag and no chemical shifts for the tag residues are reported. First VirB7 amino acid is S5 which in the proper sequence is residue number 17 as given in the chemical shift list.

1   GLYALAMETALASERGLYHISLYSPROPRO
2   PROGLUPROASPTRPSERASNTHRVALPRO
3   VALASNLYSTHRILEPROVALASPTHRGLN
4   GLYGLYARGASNGLUSER

Entity 2, VirB9 126 residues - 14060 Da.

GIDPFTHM is part of the tag and no chemical shifts for the tag residues are reported. First VirB9 amino acid is A9 which in the proper sequence is residue number 177 as given in the chemical shift list.

1   GLYILEASPPROPHETHRHISMETALAGLY
2   ALALYSASNTYRGLNTYRVALMETSERGLU
3   GLNPROGLUMETARGSERILEGLNPROVAL
4   HISVALTRPASPASNTYRARGPHETHRARG
5   PHEGLUPHEPROALAASNALAGLULEUPRO
6   GLNVALTYRMETILESERALASERGLYLYS
7   GLUTHRLEUPROASNSERHISVALVALGLY
8   GLUASNARGASNILEILEGLUVALGLUTHR
9   VALALALYSGLUTRPARGILEARGLEUGLY
10   ASPLYSVALVALGLYVALARGASNASNASN
11   PHEALAPROGLYALAGLYALAVALALATHR
12   GLYTHRALASERPROASPVALARGARGVAL
13   GLNILEGLYGLUASPASN

Samples:

sample_1: VirB7, [U-15N], 1 mM; VirB9 1 mM

sample_2: VirB7, [U-13C; U-15N], 1 mM; VirB9 1 mM

sample_3: VirB7 1 mM; VirB9, [U-15N], 1 mM

sample_4: VirB7 1 mM; VirB9, [U-13C; U-15N], 1 mM

conditions_1: pH: 6.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H 15N HSQCnot availablenot availableconditions_1
1H 13C HSQCnot availablenot availableconditions_1
1H 13C CT-HSQCnot availablenot availableconditions_1
1H 15N TOCSY-HSQCnot availablenot availableconditions_1
1H 15N NOESY-HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HN(CO)CAnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
HA(CA)NHnot availablenot availableconditions_1
HA(CACO)NHnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
HCCH-COSYnot availablenot availableconditions_1
1H 13C NOESY-HSQCnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity Plus 500 MHz
  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAF38206 BAF93109 BAM29017
EMBL CCA62524 CCE73953 CCN79904 CDF32038 CDK67343
GB AAA86458 AAL13392 ABG33818 ABG49204 ABO42055
REF NP_511196 WP_000735066 WP_000735067 WP_010999963 WP_013279390

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks