BMRB Entry 6879

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignment for aKv1.1N
Deposition date:
2005-10-27
Original release date:
2006-02-17
Authors:
Baker, Kent; Hilty, Christian; Peti, Wolfgang; Pfaffinger, Paul; Wider, Gerhard; Wuthrich, Kurt; Choe, Senyon
Citation:

Citation: Baker, Kent; Hilty, Christian; Peti, Wolfgang; Pfaffinger, Paul; Wider, Gerhard; Wuthrich, Kurt; Choe, Senyon. "NMR-Derived Dynamic Aspects of N-Type Inactivation of a Kv Channel Suggest a Transient Interaction with the T1 Domain"  Biochemistry 45, 1663-1672 (2006).
PubMed: 16460013

Assembly members:

Assembly members:
aKv1.1, polymer, 181 residues, 21012 Da.

Natural source:

Natural source:   Common Name: California sea hare   Taxonomy ID: 6500   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aplysia Aplysia californica

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts142
1H chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aKv1.1N, chain 11
2aKv1.1N, chain 21
3aKv1.1N, chain 31
4aKv1.1N, chain 41

Entities:

Entity 1, aKv1.1N, chain 1 181 residues - 21012 Da.

1   METGLUVALALAMETALAGLYILEGLUGLY
2   ASNGLYGLYPROALAGLYTYRARGASPSER
3   TYRHISSERSERGLNARGPROLEULEUARG
4   SERSERASNLEUPROASNSERARGSERPHE
5   PROLYSLEUSERGLUGLUASPASNALAASN
6   GLUASNGLYMETGLYVALPROGLYSERASP
7   TYRASPCYSSERCYSGLUARGVALVALILE
8   ASNVALSERGLYLEUARGPHEGLUTHRGLN
9   LEULYSTHRLEUASNGLNPHEPROASPTHR
10   LEULEUGLYASNPROGLNLYSARGASNARG
11   TYRTYRASPPROLEUARGASNGLUTYRPHE
12   PHEASPARGASNARGPROSERPHEASPALA
13   ILELEUTYRPHETYRGLNSERGLYGLYARG
14   LEUARGARGPROVALASNVALPROLEUASP
15   VALPHESERGLUGLUILELYSPHETYRGLU
16   LEUGLYGLUASNALAPHEGLUARGTYRARG
17   GLUASPGLUGLYPHEILELYSGLUGLUGLU
18   LYSPROLEUPROGLNASNGLUPHEGLNARG
19   ARG

Samples:

sample_1: aKv1.1, [U-2H; U-13C; U-15N], 1.0 mM; Sodium Phosphate 20 mM; NaCl 50 mM

conditions_1: pH: 7.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
(15N_1H)TROSYsample_1not availableconditions_1
(1H_1H)NOESY-(15N_1N)-TROSYsample_1not availableconditions_1
TROSY-HNCAsample_1not availableconditions_1
(H)N(COCA)NHsample_1not availableconditions_1

Software:

xwinnmr, Bruker - Collection

XEASY - Processing

CARA - Processing

NMR spectrometers:

  • Bruker . 900 MHz
  • Bruker . 800 MHz
  • Bruker . 500 MHz

Related Database Links:

PDB
GB AAA27756
REF NP_001191634

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks