BMRB Entry 6751

Title:
Solution Structure of Asl1650, an Acyl Carrier Protein from Anabaena sp. PCC 7120 with a Variant Phosphopantetheinylation-Site Sequence
Deposition date:
2005-07-26
Original release date:
2005-08-22
Authors:
Johnson, Margaret; Peti, Wolfgang; Herrmann, Torsten; Wilson, Ian; Wuthrich, Kurt
Citation:

Citation: Johnson, Margaret; Peti, Wolfgang; Herrmann, Torsten; Wilson, Ian; Wuthrich, Kurt. "Solution structure of Asl1650, an acyl carrier protein from Anabaena sp. PCC 7120 with a variant phosphopantetheinylation-site sequence"  Protein Sci. 15, 1030-1041 (2006).
PubMed: 16597827

Assembly members:

Assembly members:
Asl1650, polymer, 88 residues, 10080.4 Da.

Natural source:

Natural source:   Common Name: Anabaena sp. PCC 7120   Taxonomy ID: 103690   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena sp. PCC 7120

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts360
15N chemical shifts93
1H chemical shifts647

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Asl16501

Entities:

Entity 1, Asl1650 88 residues - 10080.4 Da.

1   GLYSERHISMETLYSTHRILEGLNPROALA
2   SERVALGLUASPILEGLNSERTRPLEUILE
3   ASPGLNPHEALAGLNGLNLEUASPVALASP
4   PROASPASPILEASPMETGLUGLUSERPHE
5   ASPASNTYRASPLEUASNSERSERLYSALA
6   LEUILELEULEUGLYARGLEUGLULYSTRP
7   LEUGLYLYSGLULEUASNPROVALLEUILE
8   PHEASNTYRPROTHRILEALAGLNLEUALA
9   LYSARGLEUGLYGLULEUTYRLEU

Samples:

sample_1: Asl1650, [U-15N; U-13C], 2 mM; phosphate buffer 20 mM; NaCl 250 mM; NaN3 2 mM

sample_2: Asl1650, [U-15N], 2 mM; phosphate buffer 20 mM; NaCl 250 mM; NaN3 2 mM

sample_3: Asl1650 2 mM; phosphate buffer 20 mM; NaCl 250 mM; NaN3 2 mM

conditions_1: ionic strength: 0.25 M; pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
15N-resolved [1H,1H]-TOCSYnot availablenot availablenot available
HC(CCO)NHnot availablenot availablenot available
HC(C)H-TOCSYnot availablenot availablenot available
2D [1H,1H]-COSYnot availablenot availablenot available
2D [1H,1H]-TOCSYnot availablenot availablenot available
2D [1H,1H]-NOESYnot availablenot availablenot available
3D 15N-resolved [1H,1H]-NOESYnot availablenot availablenot available
3D 13C-resolved [1H,1H]-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAB78016
REF NP_485690 WP_010995819

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks