BMRB Entry 6728

Title:
NMR STRUCTURAL STUDIES OF A POTASSIUM CHANNEL/CHARYBDOTOXIN COMPLEX
Deposition date:
2005-07-08
Original release date:
2006-08-15
Authors:
Yu, Liping; Olejniczak, Edward
Citation:

Citation: Yu, Liping; Sun, Chaohong; Song, Danying; Shen, Jianwei; Xu, Nan; Gunasekera, Angelo; Haiduk, Philip; Olejniczak, Edward. "Nuclear Magnetic Resonance Structural Studies of a Potassium Channel-Charybdotoxin Complex"  Biochemistry 44, 15834-15841 (2005).
PubMed: 16313186

Assembly members:

Assembly members:
potassium channel, polymer, 129 residues, Formula weight is not available
Charybdotoxin, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptomyces lividans   Taxonomy ID: 1916   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces lividans

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts46
1H chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1potassium channel KcsA, chain 11
2potassium channel KcsA, chain 21
3potassium channel KcsA, chain 31
4potassium channel KcsA, chain 41
5Charybdotoxin2

Entities:

Entity 1, potassium channel KcsA, chain 1 129 residues - Formula weight is not available

1   METPROPROMETLEUSERGLYLEULEUALA
2   ARGLEUVALLYSLEULEULEUGLYARGHIS
3   GLYSERALALEUHISTRPARGALAALAGLY
4   ALAALATHRVALLEULEUVALILEVALLEU
5   LEUALAGLYSERTYRLEUALAVALLEUALA
6   GLUARGGLYALAPROGLYALAALALEUILE
7   SERTYRPROASPALALEUTRPTRPSERVAL
8   GLUTHRALATHRTHRVALGLYTYRGLYASP
9   LEUTYRPROVALTHRLEUTRPGLYARGCYS
10   VALALAVALVALVALMETVALALAGLYILE
11   THRSERTYRGLYLEUVALPHEALAALAVAL
12   ALATHRTRPPHEVALGLYARGGLUGLNGLU
13   ARGARGGLYHISPHEVALARGHISSER

Entity 2, Charybdotoxin 37 residues - Formula weight is not available

1   PCAPHETHRASNVALSERCYSTHRTHRSER
2   LYSGLUCYSTRPSERVALCYSGLNARGLEU
3   HISASNTHRSERARGGLYLYSCYSMETASN
4   LYSLYSCYSARGCYSTYRSER

Samples:

sample_1: potassium channel0.030 – 1.0 mM; Charybdotoxin mM

experimental_conditions: pH: 7.5; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
13C-edited NOESYsample_1not availableexperimental_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
BMRB 114
GenBank AAA73014
SWISS-PROT P13487