Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6602
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Citation: Sekharan, Monica; Rajagopal, Ponni; Klevit, Rachel. "Backbone 1H, 13C, and 15N Resonance Assignment of the 46 kDa Dimeric GAF A
Domain of Phosphodiesterase 5" J. Biomol. NMR 33, 75-75 (2005).
PubMed: 16222566
Assembly members:
5 GAF A, polymer, 204 residues, Formula weight is not available
35G, non-polymer, 345.205 Da.
Natural source: Common Name: House Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Data type | Count |
13C chemical shifts | 363 |
15N chemical shifts | 117 |
1H chemical shifts | 117 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PDE5 GAF A domain | 1 |
2 | cGMP | 1 |
Entity 1, PDE5 GAF A domain 204 residues - Formula weight is not available
1 | PRO | LEU | THR | PRO | PRO | ARG | PHE | ASP | SER | ASP | ||||
2 | GLU | VAL | ASP | GLN | CYS | SER | ARG | LEU | LEU | GLU | ||||
3 | LEU | VAL | LYS | ASP | ILE | SER | SER | HIS | LEU | ASP | ||||
4 | VAL | THR | ALA | LEU | CYS | HIS | LYS | ILE | PHE | LEU | ||||
5 | HIS | ILE | HIS | GLY | LEU | ILE | SER | ALA | ASP | ARG | ||||
6 | TYR | SER | LEU | PHE | LEU | VAL | CYS | GLU | ASP | SER | ||||
7 | SER | LYS | ASP | LYS | PHE | LEU | ILE | SER | ARG | LEU | ||||
8 | PHE | ASP | VAL | ALA | GLU | GLY | SER | THR | LEU | GLU | ||||
9 | GLU | ALA | SER | ASN | ASN | CYS | ILE | ARG | LEU | GLU | ||||
10 | TRP | ASN | LYS | GLY | ILE | VAL | GLY | HIS | VAL | ALA | ||||
11 | ALA | PHE | GLY | GLU | PRO | LEU | ASN | ILE | LYS | ASP | ||||
12 | ALA | TYR | GLU | ASP | PRO | ARG | PHE | ASN | ALA | GLU | ||||
13 | VAL | ASP | GLN | ILE | THR | GLY | TYR | LYS | THR | GLN | ||||
14 | SER | ILE | LEU | CYS | MET | PRO | ILE | LYS | ASN | HIS | ||||
15 | ARG | GLU | GLU | VAL | VAL | GLY | VAL | ALA | GLN | ALA | ||||
16 | ILE | ASN | LYS | LYS | SER | GLY | ASN | GLY | GLY | THR | ||||
17 | PHE | THR | GLU | LYS | ASP | GLU | LYS | ASP | PHE | ALA | ||||
18 | ALA | TYR | LEU | ALA | PHE | CYS | GLY | ILE | VAL | LEU | ||||
19 | HIS | ASN | ALA | GLN | LEU | TYR | GLU | THR | SER | LEU | ||||
20 | LEU | GLU | ASN | LYS | ARG | ASN | LEU | GLU | HIS | HIS | ||||
21 | HIS | HIS | HIS | HIS |
15N_cGMP-bound_PDE5_GAF_A_NMR_sample: PDE5 GAF A, [U-98% 15N], 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
13C-15N_cGMP-bound_PDE5_GAF_A_NMR_sample: PDE5 GAF A, [U-99% 13C; U-99% 15N], 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
2H-13C-15N_cGMP-bound_PDE5_GAF_A_NMR_sample: PDE5 GAF A, [U-99% 2H; U-99% 13C; U-98% 15N], 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Ala: PDE5 GAF A, [U-98% 15N]-Ala, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Ile: PDE5 GAF A, [U-98% 15N]-Ile, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Leu: PDE5 GAF A, [U-98% 15N]-Leu, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Val: PDE5 GAF A, [U-98% 15N]-Val, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Asp: PDE5 GAF A, [U-98% 15N]-Asp, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Glu: PDE5 GAF A, [U-98% 15N]-Glu, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Phe: PDE5 GAF A, [U-98% 15N]-Phe, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
15N_specific_labeled_amino_acid_cGMP-bound_PDE5_GAF_A_NMR_samples_Lys: PDE5 GAF A, [U-98% 15N]-Lys, 0.7 mM; cGMP 0.7 mM; sodium phosphate 25 mM; EDTA 0.1 mM; DTT 10 mM; PMSF 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%
Sample_condition_set_1: pH: 6.5; temperature: 310 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HNCA | not available | not available | Sample_condition_set_1 |
HN(CO)CA | not available | not available | Sample_condition_set_1 |
HNCACB | not available | not available | Sample_condition_set_1 |
HN(CO)CACB | not available | not available | Sample_condition_set_1 |
HNCO | not available | not available | Sample_condition_set_1 |
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