BMRB Entry 6496

Title:
Backbone 1H and 15N Chemical Shift Assignments for Chorismate Mutase from Bacillus subtilis in complex with prephenate
Deposition date:
2005-02-08
Original release date:
2005-05-24
Authors:
Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin
Citation:

Citation: Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin. "Investigation of Ligand Binding and Protein Dynamics in Bacillus subtilis Chorismate Mutase by Transverse Relaxation Optimized Spectroscopy-Nuclear Magnetic Resonance"  Biochemistry 44, 6788-6799 (2005).
PubMed: 15865424

Assembly members:

Assembly members:
BsCM, polymer, 127 residues, Formula weight is not available
PRE, non-polymer, 226.183 Da.

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Data typeCount
1H chemical shifts220
15N chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BsCM polypeptide_A1
2BsCM polypeptide_B1
3BsCM polypeptide_C1
4prephenate_A2
5prephenate_B2
6prephenate_C2

Entities:

Entity 1, BsCM polypeptide_A 127 residues - Formula weight is not available

1   METMETILEARGGLYILEARGGLYALATHR
2   THRVALGLUARGASPTHRGLUGLUGLUILE
3   LEUGLNLYSTHRLYSGLNLEULEUGLULYS
4   ILEILEGLUGLUASNHISTHRLYSPROGLU
5   ASPVALVALGLNMETLEULEUSERALATHR
6   PROASPLEUHISALAVALPHEPROALALYS
7   ALAVALARGGLULEUSERGLYTRPGLNTYR
8   VALPROVALTHRCYSMETGLNGLUMETASP
9   VALTHRGLYGLYLEULYSLYSCYSILEARG
10   VALMETMETTHRVALGLNTHRASPVALPRO
11   GLNASPGLNILEARGHISVALTYRLEUGLU
12   LYSALAVALVALLEUARGPROASPLEUSER
13   LEUTHRLYSASNTHRGLULEU

Entity 2, prephenate_A - C10 H10 O6 - 226.183 Da.

1   PRE

Samples:

TL_BsCM: BsCM, [U-2H; U-13C; U-15N], mM; .

DL-BsCM: BsCM, [U-13C; U-15N; U-<35% 2H], mM; .

15N_BsCM_PRE: BsCM, [U-95% 15N], mM; .

conditions_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
NOESY-TROSYnot availablenot availableconditions_1
TOCSY-TROSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • . . . MHz

Related Database Links:

BMRB 6494 6495
PDB
DBJ BAI85767 BAM52750 BAM58325 GAK79439
EMBL CAB14185 CCU58771 CEI57484 CEJ77906
GB AAA20861 AAA22249 ABN05305 ABN05308 ABN13175
REF NP_390150 WP_003230594 WP_004398096 WP_004398675 WP_009967606
SP P19080
AlphaFold P19080

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks