BMRB Entry 6374

Title:
NMR STRUCTURE OF THE FIRST PHD FINGER OF AUTOIMMUNE REGULATOR PROTEIN (AIRE1): INSIGHTS INTO APECED
Deposition date:
2004-11-01
Original release date:
2005-02-10
Authors:
Bottomley, Matthew; Stier, Gunter; Krasotkina, Julya; Legube, Gaelle; Simon, Bernd; Akhtar, Asifa; Sattler, Michael; Musco, Giovanna
Citation:

Citation: Bottomley, Matthew; Stier, Gunter; Pennacchini, D.; Legube, Gaelle; Simon, Bernd; Akhtar, Asifa; Sattler, Michael; Musco, Giovanna. "NMR structure of the first PHD finger of autoimmune regulator protein (AIRE1). Insights into autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) disease"  J. Biol. Chem. 280, 11505-11512 (2005).
PubMed: 15649886

Assembly members:

Assembly members:
first PHD finger of AIRE1, polymer, 66 residues, 7138 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
first PHD finger of AIRE1: GAMAQKNEDECAVCRDGGEL ICCDGCPRAFHLACLSPPLR EIPSGTWRCSSCLQATVQEV QPRAEE

Data typeCount
13C chemical shifts201
15N chemical shifts61
1H chemical shifts649
residual dipolar couplings42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AIRE1-PHD1 monomer1
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, AIRE1-PHD1 monomer 66 residues - 7138 Da.

1   GLYALAMETALAGLNLYSASNGLUASPGLU
2   CYSALAVALCYSARGASPGLYGLYGLULEU
3   ILECYSCYSASPGLYCYSPROARGALAPHE
4   HISLEUALACYSLEUSERPROPROLEUARG
5   GLUILEPROSERGLYTHRTRPARGCYSSER
6   SERCYSLEUGLNALATHRVALGLNGLUVAL
7   GLNPROARGALAGLUGLU

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: first PHD finger of AIRE1, [U-13C; U-15N], 1 mM; NaCl 150 mM; phosphate 20 mM; DTT 5 mM

Ex-cond_1: ionic strength: 0.15 M; pH*: 6.3; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
CBCANHsample_1not availableEx-cond_1
3D HCCONH-TOCSYsample_1not availableEx-cond_1
CCONH-TOCSYsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
2D 1H-15N HMQCsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

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